WB
Western blot analysis of UCP1 on different lysates with Rabbit anti-UCP1 antibody at 1/2,000 dilution. Lane 1: Mouse brown adipose tissue lysate, Lane 2: Rat brown adipose tissue lysate, Lane 3: Rat white adipose tissue lysate (negative), Lysates/proteins at 20 µg/Lane. Exposure time: 3 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue with Rabbit anti-UCP1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse white adipose tissue (negative) with Rabbit anti-UCP1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue with Rabbit anti-UCP1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat white adipose tissue (negative) with Rabbit anti-UCP1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-F
Species: Mouse, Site: brown adipose, Sample: Frozen section, Antibody concentration: 1/500, Antigen retrieval: Not required.IP
UCP1 was immunoprecipitated from 0.2 mg mouse brown adipose tissue lysate with Rabbit anti-UCP1 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-UCP1 antibody at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: mouse brown adipose tissue lysate (input), Lane 2: Rabbit anti-UCP1 antibody IP in mouse brown adipose tissue lysate, Lane 3: Rabbit IgG instead of Rabbit anti-UCP1 antibody in mouse brown adipose tissue lysate, Blocking/Dilution buffer: primary antibody dilution, Exposure time: 3 seconds.| Product Name | UCP1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIHCIPWB |
| WB:1:2000 IHC:1:1000 IF-F:1:500 IP:1-2μg/sample |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Ucp1 |
|---|---|
| Gene Synonyms | Ucp Slc25a7 |
| Gene Full Name | uncoupling protein 1 |
| Gene Summary | Enables long-chain fatty acid binding activity; oxidative phosphorylation uncoupler activity; and purine ribonucleotide binding activity. Involved in several processes, including cellular response to cold; diet induced thermogenesis; and positive regulation of cold-induced thermogenesis. Acts upstream of or within brown fat cell differentiation and regulation of transcription by RNA polymerase II. Located in mitochondrial inner membrane. Is expressed in several structures, including adipose tissue; adrenal gland; submandibular gland; telencephalon; and thymus. Human ortholog(s) of this gene implicated in hypertension and type 2 diabetes mellitus. Orthologous to human UCP1 (uncoupling protein 1). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | mitochondrial brown fat uncoupling protein antibody Mitochondrial brown fat uncoupling protein 1 antibody SLC25A7 antibody Solute carrier family 25 member 7 antibody Thermogenin antibody UCP 1 antibody UCP antibody UCP1 antibody UCP1_HUMAN antibody uncoupling protein 1 (mitochondrial proton carrier) antibody mitochondrial brown fat uncoupling protein antibody Mitochondrial brown fat uncoupling protein 1 antibody SLC25A7 antibody Solute carrier family 25 member 7 antibody Thermogenin antibody UCP 1 antibody UCP antibody UCP1 antibody UCP1_HUMAN antibody uncoupling protein 1 (mitochondrial proton carrier) antibody Uncoupling protein 1 antibody |
| Molecular Weight(MW) | 33kDa |
| Cellular Localization | Mitochondrion inner membrane. |
WB
Western blot analysis of UCP1 on different lysates with Rabbit anti-UCP1 antibody at 1/2,000 dilution. Lane 1: Mouse brown adipose tissue lysate, Lane 2: Rat brown adipose tissue lysate, Lane 3: Rat white adipose tissue lysate (negative), Lysates/proteins at 20 µg/Lane. Exposure time: 3 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue with Rabbit anti-UCP1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse white adipose tissue (negative) with Rabbit anti-UCP1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue with Rabbit anti-UCP1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat white adipose tissue (negative) with Rabbit anti-UCP1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-F
Species: Mouse, Site: brown adipose, Sample: Frozen section, Antibody concentration: 1/500, Antigen retrieval: Not required.
IP
UCP1 was immunoprecipitated from 0.2 mg mouse brown adipose tissue lysate with Rabbit anti-UCP1 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-UCP1 antibody at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: mouse brown adipose tissue lysate (input), Lane 2: Rabbit anti-UCP1 antibody IP in mouse brown adipose tissue lysate, Lane 3: Rabbit IgG instead of Rabbit anti-UCP1 antibody in mouse brown adipose tissue lysate, Blocking/Dilution buffer: primary antibody dilution, Exposure time: 3 seconds.| Application Notes | WB:1:2000 IHC:1:1000 IF-F:1:500 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
