WB
Western blot analysis of IL-13R alpha 2 on different lysates with Rabbit anti-IL-13R alpha 2 antibody at 1/2,000 dilution. Lane 1: A375 cell lysate (no heat), Lane 2: U-937 cell lysate (no heat) (negative), Lane 3: Daudi cell lysate (negative), Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds;; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-IL-13R alpha 2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.FC
Flow cytometric analysis of A375 (positive) and U-937 (negative) cells labeling IL-13R alpha 2. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | IL-13R alpha 2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human Spermine synthase aa 100-150/366. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:2000 IHC:1:1000 FC:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | IL13RA2 |
|---|---|
| Gene Synonyms | CT19 IL-13R IL13BP CD213A2 |
| Gene Full Name | interleukin 13 receptor subunit alpha 2 |
| Gene Summary | The protein encoded by this gene is closely related to Il13RA1, a subuint of the interleukin 13 receptor complex. This protein binds IL13 with high affinity, but lacks cytoplasmic domain, and does not appear to function as a signal mediator. It is reported to play a role in the internalization of IL13. [provided by RefSeq, Jul 2008] |
| Alternative Names | Cancer/testis antigen 19 antibody CD213a2 antibody CT19 antibody I13R2_HUMAN antibody IL-13 receptor subunit alpha-2 antibody IL-13R subunit alpha-2 antibody IL-13R-alpha-2 antibody IL-13RA2 antibody IL13 receptor antibody IL13BP antibody Cancer/testis antigen 19 antibody CD213a2 antibody CT19 antibody I13R2_HUMAN antibody IL-13 receptor subunit alpha-2 antibody IL-13R subunit alpha-2 antibody IL-13R-alpha-2 antibody IL-13RA2 antibody IL13 receptor antibody IL13BP antibody IL13R antibody Il13ra2 antibody Interleukin 13 binding protein antibody Interleukin 13 receptor alpha 2 chain antibody Interleukin-13 receptor subunit alpha-2 antibody Interleukin-13-binding protein antibody |
| Molecular Weight(MW) | 44kDa(Observed band size: 50-80kDa) |
| Cellular Localization | Membrane. |
WB
Western blot analysis of IL-13R alpha 2 on different lysates with Rabbit anti-IL-13R alpha 2 antibody at 1/2,000 dilution. Lane 1: A375 cell lysate (no heat), Lane 2: U-937 cell lysate (no heat) (negative), Lane 3: Daudi cell lysate (negative), Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds;; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-IL-13R alpha 2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
FC
Flow cytometric analysis of A375 (positive) and U-937 (negative) cells labeling IL-13R alpha 2. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:2000 IHC:1:1000 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
