WB
Western blot analysis of lysates from HeLa cells treated with TSA 400nM 24H and Jurkat cells treated with forskolin 40nM 30', using Rho/Rac Guanine Nucleotide Exchange Factor 2 (Phospho-Ser885) Antibody. The lane on the right is blocked with the phospho peptide.WB
Western Blot analysis of 1 A.431 treated with LPS, 2 .A431,using primary antibody at 1:1000 dilution. Secondary antibody was diluted at 1:10000.IHC
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.| Product Name | Lfc (Phospho Ser885) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Rho/Rac Guanine Nucleotide Exchange Factor 2 around the phosphorylation site of Ser885. AA range:851-900. |
| Modification | p-Ser885 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:300 IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ARHGEF2 |
|---|---|
| Gene Synonyms | GEF Lfc P40 GEFH1 LFP40 GEF-H1 NEDMHM |
| Gene Full Name | Rho/Rac guanine nucleotide exchange factor 2 |
| Gene Summary | Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Jun 2009] |
| Alternative Names | ARHGEF2 KIAA0651 LFP40 Rho guanine nucleotide exchange factor 2 Guanine nucleotide exchange factor H1 GEF-H1 Microtubule-regulated Rho-GEF Proliferating cell nucleolar antigen p40 |
| Molecular Weight(MW) | 111kDa |
| Cellular Localization | Cytoplasm, cytoskeleton . Cytoplasm . Cell junction, tight junction . Golgi apparatus . Cytoplasm, cytoskeleton, spindle . Cell projection, ruffle membrane . Cytoplasmic vesicle . Localizes to the tips of cortical microtubules of the mitotic spindle during cell division, and is further released upon microtubule depolymerization (PubMed:15827085). Recruited into membrane ruffles induced |
WB
Western blot analysis of lysates from HeLa cells treated with TSA 400nM 24H and Jurkat cells treated with forskolin 40nM 30', using Rho/Rac Guanine Nucleotide Exchange Factor 2 (Phospho-Ser885) Antibody. The lane on the right is blocked with the phospho peptide.
WB
Western Blot analysis of 1 A.431 treated with LPS, 2 .A431,using primary antibody at 1:1000 dilution. Secondary antibody was diluted at 1:10000.
IHC
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C). |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
