WB
Western blot analysis of lysates from HCT 116 cells using Acetyl-FOXO1-K262/K265/K274 Rabbit pAb at 1:400 dilution. HCT 116 cells were treated with H2O2(0.5mM) at 37℃ for 12 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 45s.| Product Name | Acetyl-FOXO1-K262/K265/K274 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FOXO1 |
|---|---|
| Gene Synonyms | FKH1, FKHR, FOXO1A |
| Gene Full Name | forkhead box O1 |
| Gene Summary | This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008] |
| Alternative Names | FKH1 FKHR FOXO1A Acetyl-FOXO1-K262/K265/K274 |
| Molecular Weight(MW) | 70kDa(Observed MW 75kDa) |
WB
Western blot analysis of lysates from HCT 116 cells using Acetyl-FOXO1-K262/K265/K274 Rabbit pAb at 1:400 dilution. HCT 116 cells were treated with H2O2(0.5mM) at 37℃ for 12 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 45s.| Application Notes | WB:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
