WB
Positive WB detected in: Mouse skeletal muscle tissue, All lanes: SPHK1 antibody at 3μg/ml, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.IHC
Immunohistochemistry of paraffin-embedded human liver cancer using SPHK1 Antibody at dilution of 1:100.IHC
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using SPHK1 Antibody at dilution of 1:100.ICC/IF
Immunofluorescent analysis of HepG2 cells using SPHK1 Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-SPHK1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Sphingosine kinase 1 protein (1-384AA). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:5000 IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | SPHK1 |
|---|---|
| Gene Synonyms | SPHK |
| Gene Full Name | sphingosine kinase 1 |
| Gene Summary | The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Phosphorylation of this protein alters its catalytic activity and promotes its translocation to the plasma membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2017] |
| Alternative Names | SK 1 antibody SK1 antibody Sphingosine kinase 1 antibody SPHK 1 antibody SPHK antibody Sphk1 antibody SPHK1_HUMAN antibody SPK antibody SPK 1 antibody |
| Molecular Weight(MW) | 43 kDa |
| Cellular Localization | Cytoplasm. Nucleus. Cell membrane. Endosome membrane; Peripheral membrane protein. Membrane, clathrin-coated pit. Cell junction, synapse. |
WB
Positive WB detected in: Mouse skeletal muscle tissue, All lanes: SPHK1 antibody at 3μg/ml, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.
IHC
Immunohistochemistry of paraffin-embedded human liver cancer using SPHK1 Antibody at dilution of 1:100.
IHC
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using SPHK1 Antibody at dilution of 1:100.
ICC/IF
Immunofluorescent analysis of HepG2 cells using SPHK1 Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:2000-1:5000 IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300,Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
