WB
Western blot analysis of anti- PTRF/CAVIN1 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse lung tissue lysates. Use rabbit anti- PTRF/CAVIN1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit.IHC
IHC analysis of PTRF/CAVIN1 using anti-PTRF/CAVIN1 antibody. PTRF/CAVIN1 was detected in a paraffin-embedded section of human thyroid cancer tissue. The tissue section was incubated with rabbit anti-PTRF/CAVIN1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of PTRF/CAVIN1 using anti-PTRF/CAVIN1 antibody. PTRF/CAVIN1 was detected in a paraffin-embedded section of mouse lung tissue. The tissue section was incubated with rabbit anti-PTRF/CAVIN1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of PTRF/CAVIN1 using anti-PTRF/CAVIN1 antibody. PTRF/CAVIN1 was detected in a paraffin-embedded section of rat lung tissue. The tissue section was incubated with rabbit anti-PTRF/CAVIN1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
IF analysis of PTRF/CAVIN1 using anti-PTRF/CAVIN1 antibody. PTRF/CAVIN1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-PTRF/CAVIN1 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of SiHa cells using anti-PTRF/CAVIN1 antibody. Overlay histogram showing SiHa cells stained with anti-PTRF/CAVIN1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTRF/CAVIN1 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to PTRF/CAVIN1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human PTRF/CAVIN1 recombinant protein (Position: D47-D390). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CAVIN1 |
|---|---|
| Gene Synonyms | CGL4 PTRF CAVIN FKSG13 cavin-1 |
| Gene Full Name | caveolae associated protein 1 |
| Gene Summary | This gene encodes a protein that enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts. This protein regulates rRNA transcription by promoting the dissociation of transcription complexes and the reinitiation of polymerase I on nascent rRNA transcripts. This protein also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins. This protein translocates from caveolae to the cytoplasm after insulin stimulation. Caveolae contain truncated forms of this protein and may be the site of phosphorylation-dependent proteolysis. This protein is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrophy. [provided by RefSeq, Nov 2009] |
| Alternative Names | Cavin 1 FKSG13 PTRF |
| Molecular Weight(MW) | 43 kDa(Observed MW 50-55 kDa) |
| Cellular Localization | Cell membrane,Cytoplasm,Endoplasmic reticulum,Membrane,Microsome,Mitochondrion,Nucleus |
WB
Western blot analysis of anti- PTRF/CAVIN1 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse lung tissue lysates. Use rabbit anti- PTRF/CAVIN1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit.
IHC
IHC analysis of PTRF/CAVIN1 using anti-PTRF/CAVIN1 antibody. PTRF/CAVIN1 was detected in a paraffin-embedded section of human thyroid cancer tissue. The tissue section was incubated with rabbit anti-PTRF/CAVIN1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of PTRF/CAVIN1 using anti-PTRF/CAVIN1 antibody. PTRF/CAVIN1 was detected in a paraffin-embedded section of mouse lung tissue. The tissue section was incubated with rabbit anti-PTRF/CAVIN1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of PTRF/CAVIN1 using anti-PTRF/CAVIN1 antibody. PTRF/CAVIN1 was detected in a paraffin-embedded section of rat lung tissue. The tissue section was incubated with rabbit anti-PTRF/CAVIN1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
IF analysis of PTRF/CAVIN1 using anti-PTRF/CAVIN1 antibody. PTRF/CAVIN1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-PTRF/CAVIN1 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of SiHa cells using anti-PTRF/CAVIN1 antibody. Overlay histogram showing SiHa cells stained with anti-PTRF/CAVIN1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTRF/CAVIN1 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
