WB
Western blot analysis of ACPP/ACP3 using anti-ACPP/ACP3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ACPP/ACP3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of ACPP/ACP3 using anti-ACPP/ACP3 antibody. ACPP/ACP3 was detected in a paraffin-embedded section of human Prostate cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ACPP/ACP3 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of ACPP/ACP3 using anti-ACPP/ACP3 antibody. ACPP/ACP3 was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ACPP/ACP3 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of ACPP/ACP3 using anti-ACPP/ACP3 antibody. ACPP/ACP3 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ACPP/ACP3 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
IF analysis of ACPP/ACP3 using anti-ACPP/ACP3 antibody. ACPP/ACP3 was detected in an immunocytochemical section of U87 cells. The section was incubated with rabbit anti-ACPP/ACP3 Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI(Blue).FC
Flow Cytometry analysis of HL-60 cells using anti-ACPP/ACP3 antibody. Overlay histogram showing HL-60 cells stained with anti-ACPP/ACP3 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACPP/ACP3 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to ACPP/ACP3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human PAP/ACP3 recombinant protein (Position: E150-D386). Human ACP3 shares 81.4% and 80.2% amino acid (aa) sequence identity with mouse and rat ACP3, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACP3 |
|---|---|
| Gene Synonyms | ACPP 5'-NT ACP-3 TM-PAP |
| Gene Full Name | acid phosphatase 3 |
| Gene Summary | This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. [provided by RefSeq, Sep 2008] |
| Alternative Names | acid phosphatase prostate ACP 3 ACP3 ACPP PAP Prostatic acid phosphatase |
| Molecular Weight(MW) | 45 kDa(Observed MW 50 kDa) |
| Cellular Localization | Amyloid,Cell membrane,Cytoplasm,Lysosome,Membrane,Nucleus,Secreted |
WB
Western blot analysis of ACPP/ACP3 using anti-ACPP/ACP3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ACPP/ACP3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of ACPP/ACP3 using anti-ACPP/ACP3 antibody. ACPP/ACP3 was detected in a paraffin-embedded section of human Prostate cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ACPP/ACP3 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of ACPP/ACP3 using anti-ACPP/ACP3 antibody. ACPP/ACP3 was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ACPP/ACP3 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of ACPP/ACP3 using anti-ACPP/ACP3 antibody. ACPP/ACP3 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ACPP/ACP3 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
IF analysis of ACPP/ACP3 using anti-ACPP/ACP3 antibody. ACPP/ACP3 was detected in an immunocytochemical section of U87 cells. The section was incubated with rabbit anti-ACPP/ACP3 Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI(Blue).
FC
Flow Cytometry analysis of HL-60 cells using anti-ACPP/ACP3 antibody. Overlay histogram showing HL-60 cells stained with anti-ACPP/ACP3 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACPP/ACP3 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
