WB
Various lysates were subjected to SDS PAGE followed by western blot with Kindlin 1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.WB
mouse kidney tissue were subjected to SDS PAGE followed by western blot with FERMT1 Antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using FERMT1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using Kindlin 1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using FERMT1 Antibody at dilution of 1:50 (under 40x lens).IHC
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using FERMT1 Antibody at dilution of 1:50 (under 40x lens).ICC/IF
Immunofluorescent analysis of HEK-293 cells using FERMT1 antibody at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).| Product Name | Kindlin 1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human Kindlin 1 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:300-1:1000 IHC:1:300-1:1000 ICC/IF:1:10-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FERMT1 |
|---|---|
| Gene Synonyms | URP1 KIND1 DTGCU2 UNC112A C20orf42 |
| Gene Full Name | FERM domain containing kindlin 1 |
| Gene Summary | This gene encodes a member of the fermitin family, and contains a FERM domain and a pleckstrin homology domain. The encoded protein is involved in integrin signaling and linkage of the actin cytoskeleton to the extracellular matrix. Mutations in this gene have been linked to Kindler syndrome. [provided by RefSeq, Dec 2009] |
| Alternative Names | FERMT1 KIND1 Kindlerin Kindlin 1 Kindlin1 Kindlin-1 UNC112A Unc-112-related protein 1 URP1 C20orf42 DTGCU2 |
| Molecular Weight(MW) | 70-77kDa |
| Cellular Localization | Cell junction,Cell membrane,Cell projection,Cytoplasm,Cytoskeleton,Membrane |
WB
Various lysates were subjected to SDS PAGE followed by western blot with Kindlin 1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB
mouse kidney tissue were subjected to SDS PAGE followed by western blot with FERMT1 Antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using FERMT1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using Kindlin 1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using FERMT1 Antibody at dilution of 1:50 (under 40x lens).
IHC
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using FERMT1 Antibody at dilution of 1:50 (under 40x lens).
ICC/IF
Immunofluorescent analysis of HEK-293 cells using FERMT1 antibody at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:300-1:1000 IHC:1:300-1:1000 ICC/IF:1:10-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
