WB
Various lysates were subjected to SDS PAGE followed by western blot with NCKAP1 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using NAP1 Antibody at dilution of 1:50 (under 40x lens).IHC
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using NAP1 Antibody at dilution of 1:50 (under 40x lens).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using NCKAP1 antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).IF-P
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NCKAP1 antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).| Product Name | NCKAP1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human NAP1;NCKAP1 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:2000-1:16000 IHC:1:20-1:200 ICC/IF:1:200-1:800 IF-P:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NCKAP1 |
|---|---|
| Gene Synonyms | HEM2 NAP1 NAP125 p125Nap1 |
| Gene Full Name | NCK associated protein 1 |
| Gene Summary | Contributes to small GTPase binding activity. Involved in Rac protein signal transduction; positive regulation of Arp2/3 complex-mediated actin nucleation; and positive regulation of lamellipodium assembly. Located in extracellular exosome and focal adhesion. Part of SCAR complex. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | HEM2 KIAA0587 Membrane-associated protein HEM-2 NAP 1 NAP1 |
| Molecular Weight(MW) | 115-125kDa |
| Cellular Localization | Cell membrane,Cell projection,Membrane. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with NCKAP1 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using NAP1 Antibody at dilution of 1:50 (under 40x lens).
IHC
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using NAP1 Antibody at dilution of 1:50 (under 40x lens).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using NCKAP1 antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).
IF-P
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NCKAP1 antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).| Application Notes | WB:1:2000-1:16000 IHC:1:20-1:200 ICC/IF:1:200-1:800 IF-P:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
