WB
Western blot analysis of SIK2 using anti-SIK2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse 3T3-L1 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SIK2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of SIK2 using anti-SIK2 antibody SIK2 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-SIK2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of SIK2 using anti-SIK2 antibody. SIK2 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-SIK2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
IF analysis of SIK2 using anti-SIK2 antibody and anti-Beta Tubulin antibody. SIK2 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-SIK2 Antibody at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.IF-P
IF analysis of SIK2 using anti-SIK2 antibody. SIK2 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-SIK2 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of Caco-2 cells using anti-SIK2 antibody. Overlay histogram showing Caco-2 cells stained with anti-SIK2 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SIK2 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to SIK2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human SIK2 recombinant protein (Position: Q278-H919). Human SIK2 shares 88.2% amino acid (aa) sequence identity with mouse SIK2.. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIK2 |
|---|---|
| Gene Synonyms | QIK SIK-2 SNF1LK2 LOH11CR1I |
| Gene Full Name | salt inducible kinase 2 |
| Gene Summary | Enables ATP binding activity; magnesium ion binding activity; and protein kinase activity. Involved in intracellular signal transduction and protein autophosphorylation. Is active in endoplasmic reticulum membrane. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | SIK 2 salt inducible kinase 2 Qin induced kinase QIK LOH11CR1I |
| Molecular Weight(MW) | 104kDa(Observed MW 120kDa) |
| Cellular Localization | Cytoplasm,Endoplasmic reticulum membrane. |
WB
Western blot analysis of SIK2 using anti-SIK2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse 3T3-L1 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SIK2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of SIK2 using anti-SIK2 antibody SIK2 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-SIK2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of SIK2 using anti-SIK2 antibody. SIK2 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-SIK2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
IF analysis of SIK2 using anti-SIK2 antibody and anti-Beta Tubulin antibody. SIK2 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-SIK2 Antibody at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.
IF-P
IF analysis of SIK2 using anti-SIK2 antibody. SIK2 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-SIK2 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of Caco-2 cells using anti-SIK2 antibody. Overlay histogram showing Caco-2 cells stained with anti-SIK2 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SIK2 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
