WB
Western blot analysis of ALDH1A3 using anti-ALDH1A3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ALDH1A3 polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of ALDH1A3 using anti-ALDH1A3 antibody. ALDH1A3 was detected in a paraffin-embedded section of human prostate cancer tissue. The tissue section was incubated with rabbit anti-ALDH1A3 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of ALDH1A3 using anti-ALDH1A3 antibody. ALDH1A3 was detected in a paraffin-embedded section of rat lung tissue. The tissue section was incubated with rabbit anti-ALDH1A3 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
IF analysis of ALDH1A3 using anti-ALDH1A3 antibody. ALDH1A3 was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-ALDH1A3 Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of PC-3 cells using anti-ALDH1A3 antibody. Overlay histogram showing PC-3 cells stained with anti-ALDH1A3 antibody (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALDH1A3 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to ALDH1A3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human ALDH1A3 recombinant protein (Position: N37-K154). Human ALDH1A3 shares 89% and 87.3% amino acid (aa) sequence identity with mouse and rat ALDH1A3, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ALDH1A3 |
|---|---|
| Gene Synonyms | ALDH6 MCOP8 RALDH3 ALDH1A6 |
| Gene Full Name | aldehyde dehydrogenase 1 family member A3 |
| Gene Summary | This gene encodes an aldehyde dehydrogenase enzyme that uses retinal as a substrate. Mutations in this gene have been associated with microphthalmia, isolated 8, and expression changes have also been detected in tumor cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014] |
| Alternative Names | Aldehyde dehydrogenase 6 ALDH1A3 ALDH1A6 ALDH6 RALDH 3 RALDH3 Retinaldehyde dehydrogenase 3 |
| Molecular Weight(MW) | 56kDa |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of ALDH1A3 using anti-ALDH1A3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ALDH1A3 polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of ALDH1A3 using anti-ALDH1A3 antibody. ALDH1A3 was detected in a paraffin-embedded section of human prostate cancer tissue. The tissue section was incubated with rabbit anti-ALDH1A3 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of ALDH1A3 using anti-ALDH1A3 antibody. ALDH1A3 was detected in a paraffin-embedded section of rat lung tissue. The tissue section was incubated with rabbit anti-ALDH1A3 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
IF analysis of ALDH1A3 using anti-ALDH1A3 antibody. ALDH1A3 was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-ALDH1A3 Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of PC-3 cells using anti-ALDH1A3 antibody. Overlay histogram showing PC-3 cells stained with anti-ALDH1A3 antibody (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALDH1A3 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
