WB
Various lysates were subjected to SDS PAGE followed by western blot with CYR61/CCN1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using CYR61/CCN1 antibody at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using CYR61/CCN1 antibody at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CYR61/CCN1 antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).FC
1x10^6 HeLa cells were intracellularly stained with 0.25 ug CYR61/CCN1 Polyclonal antibody and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb(blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.IP
IP result of anti-CYR61/CCN1 (IP:4ug; Detection:1:800) with HeLa cells lysate 1200 ug.| Product Name | CYR61/CCN1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human CYR61 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:1000-1:4000 IHC:1:50-1:500 ICC/IF:1:200-1:800 FC:0.25 ug per 10^6 cells in a 100 µl suspension IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.75mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCN1 |
|---|---|
| Gene Synonyms | GIG1 CYR61 IGFBP10 |
| Gene Full Name | cellular communication network factor 1 |
| Gene Summary | The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation. [provided by RefSeq, Sep 2011] |
| Alternative Names | CYR61 CCN family member 1 CCN1 Cellular communication network factor 1 Cysteine-rich angiogenic inducer 61 |
| Molecular Weight(MW) | 42 kDa |
| Cellular Localization | Secreted. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with CYR61/CCN1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using CYR61/CCN1 antibody at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using CYR61/CCN1 antibody at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CYR61/CCN1 antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).
FC
1x10^6 HeLa cells were intracellularly stained with 0.25 ug CYR61/CCN1 Polyclonal antibody and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb(blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
IP
IP result of anti-CYR61/CCN1 (IP:4ug; Detection:1:800) with HeLa cells lysate 1200 ug.| Application Notes | WB:1:1000-1:4000 IHC:1:50-1:500 ICC/IF:1:200-1:800 FC:0.25 ug per 10^6 cells in a 100 µl suspension IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
