WB
Positive WB detected in: Rat brain tisseu, Rat heart tissue. All lanes: RBM45 antibody at 12μg/ml, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.ICC/IF
Immunofluorescence staining of HepG2 cells withRBM45 antibody at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-RBM45 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human RNA-binding protein 45 protein (170-244AA). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:5000 ICC/IF:1:25-1:200 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | RBM45 |
|---|---|
| Gene Synonyms | DRB1 RB-1 |
| Gene Full Name | RNA binding motif protein 45 |
| Gene Summary | This gene encodes a member of the RNA recognition motif (RRM)-type RNA-binding family of proteins. This protein exhibits preferential binding to poly(C) RNA. Initial cloning of this gene found that the rat ortholog was dynamically expressed in the developing rat brain. This protein has been localized to inclusion bodies in the brain and spinal cord of amyotrophic lateral sclerosis and Alzheimer's patients. A pseudogene has been identified on chromosome 8. [provided by RefSeq, Feb 2015] |
| Alternative Names | Developmentally-regulated RNA-binding protein 1 antibody DRB1 antibody FLJ44612 antibody MGC42237 antibody Putative RNA binding protein RB 1 antibody RB-1 antibody RBM45 antibody RBM45_HUMAN antibody RNA-binding motif protein 45 antibody RNA-binding protein 45 antibody |
| Molecular Weight(MW) | 54kDa |
| Cellular Localization | Cytoplasm. Nucleus. Note:Predominantly cytoplasmic. May shuttle between cytoplasm and nucleus. |
WB
Positive WB detected in: Rat brain tisseu, Rat heart tissue. All lanes: RBM45 antibody at 12μg/ml, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.
ICC/IF
Immunofluorescence staining of HepG2 cells withRBM45 antibody at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:5000 ICC/IF:1:25-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300,Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
