WB
Western blot analysis of TRPV4 on different lysates with Rabbit anti-TRPV4 antibody at 1/1,000 dilution. Lane 1: A375 cell lysate, Lane 2: 293T cell lysate (negative), Lysates/proteins at 20 µg/Lane. Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-TRPV4 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-F
Species: Mouse, Site: kidney, Sample: Frozen section, Antibody concentration: 1/500, Antigen retrieval: Not required.IP
TRPV4 was immunoprecipitated from 0.2 mg A375 cell lysate with Rabbit anti-TRPV4 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-TRPV4 antibody at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A375 cell lysate (input), Lane 2: Rabbit anti-TRPV4 antibody IP in A375 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-TRPV4 antibody in A375 cell lysate. Blocking/Dilution buffer: primary antibody dilution, Exposure time: 59 seconds.| Product Name | TRPV4 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIHCIPWB |
| WB:1:1000 IHC:1:1000 IF-F:1:500 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TRPV4 |
|---|---|
| Gene Synonyms | SMAL VRL2 BCYM3 CMT2C SPSMA TRP12 VROAC HMSN2C OTRPC4 SSQTL1 |
| Gene Full Name | transient receptor potential cation channel subfamily V member 4 |
| Gene Summary | This gene encodes a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. The encoded protein is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure. Mutations in this gene are the cause of spondylometaphyseal and metatropic dysplasia and hereditary motor and sensory neuropathy type IIC. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010] |
| Alternative Names | BCYM3 antibody CMT2C antibody HMSN2C antibody osm 9 like TRP channel 4 antibody Osm-9-like TRP channel 4 antibody OSM9 like transient receptor potential channel 4 antibody Osmosensitive transient receptor potential channel 4 antibody OTRPC 4 antibody OTRPC4 antibody SMAL antibody BCYM3 antibody CMT2C antibody HMSN2C antibody osm 9 like TRP channel 4 antibody Osm-9-like TRP channel 4 antibody OSM9 like transient receptor potential channel 4 antibody Osmosensitive transient receptor potential channel 4 antibody OTRPC 4 antibody OTRPC4 antibody SMAL antibody SPSMA antibody SSQTL1 antibody Transient receptor potential cation channel subfamily V member 4 antibody Transient receptor potential protein 12 antibody TRP 12 antibody TRP12 antibody TRPV 4 antibody TrpV4 antibody TRPV4_HUMAN antibody Vanilloid receptor like channel 2 antibody Vanilloid receptor like protein 2 antibody Vanilloid receptor related osmotically activated channel antibody Vanilloid receptor-like channel 2 antibody Vanilloid receptor-like protein 2 antibody Vanilloid receptor-related osmotically-activated channel antibody VR 4 antibody VR OAC antibody VR-OAC antibody VR4 antibody VRL 2 antibody VRL-2 antibody VRL2 antibody VROAC antibody |
| Molecular Weight(MW) | 98kDa(Observed band size: 90kDa) |
| Cellular Localization | Cell membrane, Apical cell membrane, Cell junction, adherens junction, Cell projection, cilium. |
WB
Western blot analysis of TRPV4 on different lysates with Rabbit anti-TRPV4 antibody at 1/1,000 dilution. Lane 1: A375 cell lysate, Lane 2: 293T cell lysate (negative), Lysates/proteins at 20 µg/Lane. Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-TRPV4 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-F
Species: Mouse, Site: kidney, Sample: Frozen section, Antibody concentration: 1/500, Antigen retrieval: Not required.
IP
TRPV4 was immunoprecipitated from 0.2 mg A375 cell lysate with Rabbit anti-TRPV4 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-TRPV4 antibody at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A375 cell lysate (input), Lane 2: Rabbit anti-TRPV4 antibody IP in A375 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-TRPV4 antibody in A375 cell lysate. Blocking/Dilution buffer: primary antibody dilution, Exposure time: 59 seconds.| Application Notes | WB:1:1000 IHC:1:1000 IF-F:1:500 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
