WB
Western blot analysis of IFNGR1 on different lysates with Rabbit anti-IFNGR1 antibody at 1/1,000 dilution. Lane 1: NCI-H226 cell lysate, Lane 2: LoVo cell lysate, Lane 3: HepG2 cell lysate, Lane 4: THP-1 cell lysate, Lane 5: MCF7 cell lysate (low expression), Lane 6: K-562 cell lysate, Lane 7: SW620 cell lysate, Lane 8: HL-60 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: Lane 1: 20 seconds; Lane 2-8: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of LoVo (positive) and MCF7 (low expression) labeling IFNGR1 with Rabbit anti-IFNGR1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IFNGR1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Product Name | IFNGR1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human IFNGR1 aa 18-245 (Extracellular). |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000 ICC/IF:1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | IFNGR1 |
|---|---|
| Gene Synonyms | CD119 IFNGR IMD27A IMD27B |
| Gene Full Name | interferon gamma receptor 1 |
| Gene Summary | This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection. [provided by RefSeq, Jul 2008] |
| Alternative Names | Antiviral Protein Type II antibody Antiviral protein type 2 antibody AVP type II antibody AVP type 2 antibody CD 119 antibody CD119 antibody CD119 antigen antibody CDw119 antibody FLJ45734 antibody IFN gamma R alpha antibody Antiviral Protein Type II antibody Antiviral protein type 2 antibody AVP type II antibody AVP type 2 antibody CD 119 antibody CD119 antibody CD119 antigen antibody CDw119 antibody FLJ45734 antibody IFN gamma R alpha antibody IFN gamma R antibody IFN gamma R1 antibody IFN-gamma receptor 1 antibody IFN-gamma-R1 antibody IFNG R1 antibody IFNGR 1 antibody IFNGR antibody IFNGR1 antibody Immune interferon receptor 1 antibody Immune interferon receptor for antibody INGR1_HUMAN antibody Interferon gamma receptor 1 antibody Interferon gamma receptor alpha chain antibody Interferon gamma receptor alpha chain precursor antibody |
| Molecular Weight(MW) | 54kDa(Observed band size: 40-100kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of IFNGR1 on different lysates with Rabbit anti-IFNGR1 antibody at 1/1,000 dilution. Lane 1: NCI-H226 cell lysate, Lane 2: LoVo cell lysate, Lane 3: HepG2 cell lysate, Lane 4: THP-1 cell lysate, Lane 5: MCF7 cell lysate (low expression), Lane 6: K-562 cell lysate, Lane 7: SW620 cell lysate, Lane 8: HL-60 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: Lane 1: 20 seconds; Lane 2-8: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of LoVo (positive) and MCF7 (low expression) labeling IFNGR1 with Rabbit anti-IFNGR1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IFNGR1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:1000 ICC/IF:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
