IP
Immunoprecipitating GAPDH in Hela whole cell lysate with rabbit anti-GAPDH antibody. Western blot was performed on the immunoprecipitate using anti-GAPDH antibody, and followed by the normal Anti-Rabbit IgG - HRP and Anti-Rabbit IgG - HRP For IP/CoIP. Lane A and D (Input): Hela whole cell lysate; Lane B and E (-): Rabbit IgG Isotype Control instead of GAPDH antibody in Hela whole cell lysate; Lane C and F (+): GAPDH antibody IP in Hela whole cell lysate.| Product Name | Mouse Anti-Rabbit IgG - HRP For IP/CoIP |
|---|---|
| Antibody Type | Secondary Antibodies |
| Immunogen | Rabbit IgG |
| Conjugation | HRP |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | IP |
| WB:1:500-1:2000 IP:1:10-1:100 |
|
| Species Reactivity | Rabbit |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
IP
Immunoprecipitating GAPDH in Hela whole cell lysate with rabbit anti-GAPDH antibody. Western blot was performed on the immunoprecipitate using anti-GAPDH antibody, and followed by the normal Anti-Rabbit IgG - HRP and Anti-Rabbit IgG - HRP For IP/CoIP. Lane A and D (Input): Hela whole cell lysate; Lane B and E (-): Rabbit IgG Isotype Control instead of GAPDH antibody in Hela whole cell lysate; Lane C and F (+): GAPDH antibody IP in Hela whole cell lysate.| Application Notes | WB:1:500-1:2000 IP:1:10-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in PBS, pH 7.4, containing 50% glycerol, 0.05% Proclin |
