WB
Western blot analysis of Phosphoserine aminotransferase on different lysates with Rabbit anti-Phosphoserine aminotransferase antibody at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate (20 µg/Lane), Lane 2: mouse brain tissue lysate (40 µg/Lane), Lane 3: rat brain tissue lysate (40 µg/Lane), Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of Phosphoserine aminotransferase on different lysates with Rabbit anti-Phosphoserine aminotransferase antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate, Lane 2: HEK-293 cell lysate, Lane 3: HepG2 cell lysate, Lane 4: K-562 cell lysate, Lane 5: A549 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Phosphoserine aminotransferase antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Phosphoserine aminotransferase antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Phosphoserine aminotransferase antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.FC
Flow cytometric analysis of HeLa cells labeling Phosphoserine aminotransferase. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).IP
Phosphoserine aminotransferase was immunoprecipitated from 0.2 mg HeLa cell lysate with Rabbit anti-Phosphoserine aminotransferase antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-Phosphoserine aminotransferase antibody at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input), Lane 2: Rabbit anti-Phosphoserine aminotransferase antibody IP in HeLa cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-Phosphoserine aminotransferase antibody in HeLa cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 2 seconds.| Product Name | Phosphoserine aminotransferase Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human PSAT1 aa 1-370. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCIPWB |
| WB:1:2000 IHC:1:1000 FC:1:1000 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PSAT1 |
|---|---|
| Gene Synonyms | PSA EPIP NLS2 PSAT PSATD |
| Gene Full Name | phosphoserine aminotransferase 1 |
| Gene Summary | This gene encodes a member of the class-V pyridoxal-phosphate-dependent aminotransferase family. The encoded protein is a phosphoserine aminotransferase and decreased expression may be associated with schizophrenia. Mutations in this gene are also associated with phosphoserine aminotransferase deficiency. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 1, 3, and 8. [provided by RefSeq, Jul 2013] |
| Alternative Names | EC 2.6.1.52 antibody Endometrial progesterone induced protein antibody EPIP antibody MGC1460 antibody NLS2 antibody Phosphohydroxythreonine aminotransferase antibody phosphoserine aminotransferase 1 antibody Phosphoserine aminotransferase antibody PSA antibody PSAT antibody EC 2.6.1.52 antibody Endometrial progesterone induced protein antibody EPIP antibody MGC1460 antibody NLS2 antibody Phosphohydroxythreonine aminotransferase antibody phosphoserine aminotransferase 1 antibody Phosphoserine aminotransferase antibody PSA antibody PSAT antibody Psat1 antibody PSATD antibody SERC_HUMAN antibody |
| Molecular Weight(MW) | 40kDa |
| Cellular Localization | Cytoplasm, cytosol. |
WB
Western blot analysis of Phosphoserine aminotransferase on different lysates with Rabbit anti-Phosphoserine aminotransferase antibody at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate (20 µg/Lane), Lane 2: mouse brain tissue lysate (40 µg/Lane), Lane 3: rat brain tissue lysate (40 µg/Lane), Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of Phosphoserine aminotransferase on different lysates with Rabbit anti-Phosphoserine aminotransferase antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate, Lane 2: HEK-293 cell lysate, Lane 3: HepG2 cell lysate, Lane 4: K-562 cell lysate, Lane 5: A549 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Phosphoserine aminotransferase antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Phosphoserine aminotransferase antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Phosphoserine aminotransferase antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
FC
Flow cytometric analysis of HeLa cells labeling Phosphoserine aminotransferase. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
IP
Phosphoserine aminotransferase was immunoprecipitated from 0.2 mg HeLa cell lysate with Rabbit anti-Phosphoserine aminotransferase antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-Phosphoserine aminotransferase antibody at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input), Lane 2: Rabbit anti-Phosphoserine aminotransferase antibody IP in HeLa cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-Phosphoserine aminotransferase antibody in HeLa cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 2 seconds.| Application Notes | WB:1:2000 IHC:1:1000 FC:1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
