WB
Western blot analysis of GATA6 using anti-GATA6 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: 293T whole cell lysates, Lane 2: MCF-7 whole cell lysates, Lane 3: PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GATA6 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.ICC/IF
IF analysis of GATA6 using anti-GATA6 antibody. GATA6 was detected in an immunocytochemical section of Caco-2 cells. The section was incubated with rabbit anti-GATA6 Antibody at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).ICC/IF
IF analysis of GATA6 using anti-GATA6 antibody. GATA6 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-GATA6 Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI(Blue).FC
Flow Cytometry analysis of U2OS cells using anti-GATA6 antibody. Overlay histogram showing U2OS cells stained with anti-GATA6 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GATA6 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to GATA6 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human GATA6 recombinant protein (Position: N105-L151). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GATA6 |
|---|---|
| Gene Full Name | GATA binding protein 6 |
| Gene Summary | This gene is a member of a small family of zinc finger transcription factors that play an important role in the regulation of cellular differentiation and organogenesis during vertebrate development. This gene is expressed during early embryogenesis and localizes to endo- and mesodermally derived cells during later embryogenesis and thereby plays an important role in gut, lung, and heart development. Mutations in this gene are associated with several congenital defects. [provided by RefSeq, Mar 2012] |
| Alternative Names | GATA binding factor 6 GATA binding protein 6 GATA6 Transcription factor GATA 6 |
| Molecular Weight(MW) | 65kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of GATA6 using anti-GATA6 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: 293T whole cell lysates, Lane 2: MCF-7 whole cell lysates, Lane 3: PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GATA6 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
ICC/IF
IF analysis of GATA6 using anti-GATA6 antibody. GATA6 was detected in an immunocytochemical section of Caco-2 cells. The section was incubated with rabbit anti-GATA6 Antibody at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
ICC/IF
IF analysis of GATA6 using anti-GATA6 antibody. GATA6 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-GATA6 Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI(Blue).
FC
Flow Cytometry analysis of U2OS cells using anti-GATA6 antibody. Overlay histogram showing U2OS cells stained with anti-GATA6 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GATA6 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
