WB
Western blot analysis of ABCB5 using anti-ABCB5 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ABCB5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of ABCB5 using anti-ABCB5 antibody. ABCB5 was detected in a paraffin-embedded section of human melanoma tissue. The tissue section was incubated with rabbit anti-ABCB5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of ABCB5 using anti-ABCB5 antibody. ABCB5 was detected in a paraffin-embedded section of mouse intestine tissue. The tissue section was incubated with rabbit anti-ABCB5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of ABCB5 using anti-ABCB5 antibody. ABCB5 was detected in a paraffin-embedded section of rat intestine tissue. The tissue section was incubated with rabbit anti-ABCB5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
IF analysis of ABCB5 using anti-ABCB5 antibody. ABCB5 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-ABCB5 Antibody at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of HL-60 cells using anti-ABCB5 antibody. Overlay histogram showing HL-60 cells stained with anti-ABCB5 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCB5 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to ABCB5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human ABCB5 recombinant protein (Position: M1-D176). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ABCB5 |
|---|---|
| Gene Synonyms | ABCB5beta EST422562 ABCB5alpha |
| Gene Full Name | ATP binding cassette subfamily B member 5 |
| Gene Summary | ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM, Mar 2008] |
| Alternative Names | ABCB5 P-gp P-glycoprotein ABCB5 ABCB5 ABCB5beta EST422562 ABCB5alpha |
| Molecular Weight(MW) | 110kDa |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of ABCB5 using anti-ABCB5 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ABCB5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of ABCB5 using anti-ABCB5 antibody. ABCB5 was detected in a paraffin-embedded section of human melanoma tissue. The tissue section was incubated with rabbit anti-ABCB5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of ABCB5 using anti-ABCB5 antibody. ABCB5 was detected in a paraffin-embedded section of mouse intestine tissue. The tissue section was incubated with rabbit anti-ABCB5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of ABCB5 using anti-ABCB5 antibody. ABCB5 was detected in a paraffin-embedded section of rat intestine tissue. The tissue section was incubated with rabbit anti-ABCB5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
IF analysis of ABCB5 using anti-ABCB5 antibody. ABCB5 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-ABCB5 Antibody at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of HL-60 cells using anti-ABCB5 antibody. Overlay histogram showing HL-60 cells stained with anti-ABCB5 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCB5 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
