WB
Positive WB detected in: MCF-7 whole cell lysate, All lanes: NCF2 antibody at 2.8µg/ml, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.IHC
Immunohistochemistry analysis of human spleen tissue using NCF2 Antibody at dilution of 1:100.IHC
Immunocytochemistry analysis of human lung tissue using NCF2 Antibody at dilution of 1:100.ICC/IF
Immunofluorescent analysis of Hela cells using NCF2 Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-NCF2 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Neutrophil cytosol factor 2 protein (178-526AA). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:5000 IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | NCF2 |
|---|---|
| Gene Synonyms | NCF-2 NOXA2 P67PHOX P67-PHOX |
| Gene Full Name | neutrophil cytosolic factor 2 |
| Gene Summary | This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010] |
| Alternative Names | 67 kDa neutrophil oxidase factor antibody Chronic granulomatous disease autosomal 2 antibody FLJ93058 antibody NADPH oxidase activator 2 antibody NCF-2 antibody Ncf2 antibody NCF2_HUMAN antibody Neutrophil cytosol factor 2 antibody Neutrophil cytosolic factor 2 (65kD chronic granulomatous disease autosomal 2) antibody Neutrophil NADPH oxidase factor 2 antibody NOXA2 antibody P67 PHOX antibody p67-phox antibody p67phox antibody |
| Molecular Weight(MW) | 51/48 kDa |
| Cellular Localization | Cytoplasm. |
WB
Positive WB detected in: MCF-7 whole cell lysate, All lanes: NCF2 antibody at 2.8µg/ml, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.
IHC
Immunohistochemistry analysis of human spleen tissue using NCF2 Antibody at dilution of 1:100.
IHC
Immunocytochemistry analysis of human lung tissue using NCF2 Antibody at dilution of 1:100.
ICC/IF
Immunofluorescent analysis of Hela cells using NCF2 Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:5000 IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
