WB
Various lysates were subjected to SDS PAGE followed by western blot with TET2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using TET2 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TET2 antibody at dilution of 1:800 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L), CL594-Phalloidin (red).FC
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TET2 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit.IP
IP result of anti-TET2 (IP:3ug; Detection:1:500) with mouse brain tissue lysate 4000ug.| Product Name | VSX2 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human TET2 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:500-1:1000 IHC:1:200-1:1000 ICC/IF:1:400-1:1600 FC:0.40 ug per 10^6 cells in a 100 µl suspension IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.8mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TET2 |
|---|---|
| Gene Synonyms | MDS IMD75 KIAA1546 |
| Gene Full Name | tet methylcytosine dioxygenase 2 |
| Gene Summary | The protein encoded by this gene is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine. The encoded protein is involved in myelopoiesis, and defects in this gene have been associated with several myeloproliferative disorders. Two variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011] |
| Alternative Names | EC:1.14.11.80 KIAA1546 Methylcytosine dioxygenase TET2 Nbla00191 |
| Molecular Weight(MW) | 130/250kDa |
| Cellular Localization | Chromosome,Nucleus. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with TET2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using TET2 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TET2 antibody at dilution of 1:800 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L), CL594-Phalloidin (red).
FC
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TET2 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit.
IP
IP result of anti-TET2 (IP:3ug; Detection:1:500) with mouse brain tissue lysate 4000ug.| Application Notes | WB:1:500-1:1000 IHC:1:200-1:1000 ICC/IF:1:400-1:1600 FC:0.40 ug per 10^6 cells in a 100 µl suspension IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
