WB
Western blot analysis of CSNK1A1 + CSNK1A1L on different lysates with Rabbit anti-CSNK1A1 + CSNK1A1L antibody at 1/2,000 dilution. Lane 1: HCT 116 cell lysate (20 µg/Lane), Lane 2: HeLa cell lysate (20 µg/Lane), Lane 3: HEK-293 cell lysate (20 µg/Lane), Lane 4: MCF7 cell lysate (20 µg/Lane), Lane 5: Jurkat cell lysate (20 µg/Lane), Lane 6: NIH/3T3 cell lysate (20 µg/Lane), Lane 7: RAW264.7 cell lysate (20 µg/Lane), Lane 8: PC-12 cell lysate (20 µg/Lane), Lane 9: Mouse brain tissue lysate (40 µg/Lane), Lane 10: Rat brain tissue lysate (40 µg/Lane), Lane 11: Mouse kidney tissue lysate (40 µg/Lane), Lane 12: Rat kidney tissue lysate (40 µg/Lane), Lane 13: Mouse spleen tissue lysate (40 µg/Lane), Lane 14: Rat spleen tissue lysate (40 µg/Lane), Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-CSNK1A1 + CSNK1A1L antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CSNK1A1 + CSNK1A1L antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-CSNK1A1 + CSNK1A1L antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-CSNK1A1 + CSNK1A1L antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-CSNK1A1 + CSNK1A1L antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of HCT 116 cells labeling CSNK1A1 + CSNK1A1L with Rabbit anti-CSNK1A1 + CSNK1A1L antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CSNK1A1 + CSNK1A1L antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of HCT 116 cells labeling CSNK1A1 + CSNK1A1L. Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).IP
CSNK1A1 + CSNK1A1L was immunoprecipitated from 0.2 mg HeLa cell lysate with Rabbit anti-CSNK1A1 + CSNK1A1L antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-CSNK1A1 + CSNK1A1L antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input), Lane 2: Rabbit anti-CSNK1A1 + CSNK1A1L antibody IP in HeLa cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-CSNK1A1 + CSNK1A1L antibody in HeLa cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 1 minute 11 seconds.| Product Name | CSNK1A1 + CSNK1A1L Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human CSNK1A1 aa 1-337 / 337. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:2000 IHC:1:1000 ICC/IF:1:500 FC:1:1000 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CSNK1A1 |
|---|---|
| Gene Synonyms | CK1 CK1a CKIa HLCDGP1 PRO2975 HEL-S-77p |
| Gene Full Name | casein kinase 1 alpha 1 |
| Gene Summary | Enables protein serine/threonine kinase activity. Involved in several processes, including intermediate filament cytoskeleton organization; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; and regulation of signal transduction. Located in cytoskeleton and nucleus. Part of beta-catenin destruction complex. Biomarker of Alzheimer's disease and inclusion body myositis. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Casein kinase 1 alpha 1 antibody Casein kinase I isoform alpha antibody CK1 antibody CK1A antibody CKI alpha antibody CKI-alpha antibody CKIa antibody Clock regulator kinase antibody Csnk1a1 antibody Down regulated in lung cancer antibody Casein kinase 1 alpha 1 antibody Casein kinase I isoform alpha antibody CK1 antibody CK1A antibody CKI alpha antibody CKI-alpha antibody CKIa antibody Clock regulator kinase antibody Csnk1a1 antibody Down regulated in lung cancer antibody HLCDGP1 antibody KC1A_HUMAN antibody PRO2975 antibody Casein kinase 1 alpha 1 like antibody casein kinase 1 alpha 1-like antibody Casein kinase I alpha S like antibody Casein kinase I isoform alpha like antibody CK1 antibody CKI alpha like antibody MGC33182 antibody OTTHUMP00000018267 antibody RP11 532O21.2 antibody |
| Molecular Weight(MW) | 39kDa(Observed band size: 34kDa) |
| Cellular Localization | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Chromosome, centromere, kinetochore, Nucleus speckle, cilium basal body, spindle. |
WB
Western blot analysis of CSNK1A1 + CSNK1A1L on different lysates with Rabbit anti-CSNK1A1 + CSNK1A1L antibody at 1/2,000 dilution. Lane 1: HCT 116 cell lysate (20 µg/Lane), Lane 2: HeLa cell lysate (20 µg/Lane), Lane 3: HEK-293 cell lysate (20 µg/Lane), Lane 4: MCF7 cell lysate (20 µg/Lane), Lane 5: Jurkat cell lysate (20 µg/Lane), Lane 6: NIH/3T3 cell lysate (20 µg/Lane), Lane 7: RAW264.7 cell lysate (20 µg/Lane), Lane 8: PC-12 cell lysate (20 µg/Lane), Lane 9: Mouse brain tissue lysate (40 µg/Lane), Lane 10: Rat brain tissue lysate (40 µg/Lane), Lane 11: Mouse kidney tissue lysate (40 µg/Lane), Lane 12: Rat kidney tissue lysate (40 µg/Lane), Lane 13: Mouse spleen tissue lysate (40 µg/Lane), Lane 14: Rat spleen tissue lysate (40 µg/Lane), Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-CSNK1A1 + CSNK1A1L antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CSNK1A1 + CSNK1A1L antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-CSNK1A1 + CSNK1A1L antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-CSNK1A1 + CSNK1A1L antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-CSNK1A1 + CSNK1A1L antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HCT 116 cells labeling CSNK1A1 + CSNK1A1L with Rabbit anti-CSNK1A1 + CSNK1A1L antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CSNK1A1 + CSNK1A1L antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of HCT 116 cells labeling CSNK1A1 + CSNK1A1L. Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
IP
CSNK1A1 + CSNK1A1L was immunoprecipitated from 0.2 mg HeLa cell lysate with Rabbit anti-CSNK1A1 + CSNK1A1L antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-CSNK1A1 + CSNK1A1L antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input), Lane 2: Rabbit anti-CSNK1A1 + CSNK1A1L antibody IP in HeLa cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-CSNK1A1 + CSNK1A1L antibody in HeLa cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 1 minute 11 seconds.| Application Notes | WB:1:2000 IHC:1:1000 ICC/IF:1:500 FC:1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
