WB
Western blot analysis of TRIM25 on different lysates with Rabbit anti-TRIM25 antibody at 1/1,000 dilution. Lane 1: HepG2 cell lysate, Lane 2: HeLa cell lysate, Lane 3: K-562 cell lysate, Lane 4: A549 cell lysate, Lane 5: MCF7 cell lysate, Lane 6: Jurkat cell lysate, Lane 7: 293T cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.IHC
Immunocytochemistry analysis of HeLa cells labeling TRIM25 with Rabbit anti-TRIM25 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TRIM25 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Product Name | TRIM25 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human TRIM25/EFP aa 100-200. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000 ICC/IF:1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TRIM25 |
|---|---|
| Gene Synonyms | EFP Z147 RNF147 ZNF147 |
| Gene Full Name | tripartite motif containing 25 |
| Gene Summary | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein is an RNA binding protein, functions as a ubiquitin E3 ligase and is involved in multiple cellular processes, including regulation of antiviral innate immunity. [provided by RefSeq, Sep 2021] |
| Alternative Names | E3 ubiquitin/ISG15 ligase TRIM25 antibody EFP antibody Estrogen responsive finger protein antibody Estrogen-responsive finger protein antibody RING finger protein 147 antibody RNF 147 antibody RNF147 antibody TRI25 antibody TRI25_HUMAN antibody TRIM 25 antibody E3 ubiquitin/ISG15 ligase TRIM25 antibody EFP antibody Estrogen responsive finger protein antibody Estrogen-responsive finger protein antibody RING finger protein 147 antibody RNF 147 antibody RNF147 antibody TRI25 antibody TRI25_HUMAN antibody TRIM 25 antibody Trim25 antibody Tripartite motif containing 25 antibody Tripartite motif containing protein 25 antibody Tripartite motif protein TRIM25 antibody Tripartite motif-containing protein 25 antibody Tripartite motifcontaining25 antibody Ubiquitin/ISG15-conjugating enzyme TRIM25 antibody Z147 antibody Zinc finger protein 147 (estrogen responsive finger protein) antibody Zinc finger protein 147 antibody Zinc finger protein-147 antibody ZNF 147 antibody ZNF147 antibody |
| Molecular Weight(MW) | 71kDa |
| Cellular Localization | Cytoplasm, Stress granule, Nucleus. |
WB
Western blot analysis of TRIM25 on different lysates with Rabbit anti-TRIM25 antibody at 1/1,000 dilution. Lane 1: HepG2 cell lysate, Lane 2: HeLa cell lysate, Lane 3: K-562 cell lysate, Lane 4: A549 cell lysate, Lane 5: MCF7 cell lysate, Lane 6: Jurkat cell lysate, Lane 7: 293T cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
IHC
Immunocytochemistry analysis of HeLa cells labeling TRIM25 with Rabbit anti-TRIM25 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TRIM25 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:1000 ICC/IF:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
