IHC
Immunohistochemistry of paraffin-embedded human pancreatic cancer using SMPD3 Antibody at dilution of 1:100.IP
Immunoprecipitating SMPD3 in mouse brain whole cell lysate, Lane 1: Rabbit control IgG (1μg) instead of SMPD3 Antibody in mouse brain whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: SMPD3 Antibody (6μg) + Mouse brain whole cell lysate (500μg), Lane 3: Mouse brain whole cell lysate (10μg).| Product Name | Rabbit anti-SMPD3 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Sphingomyelin phosphodiesterase 3 protein (401-655AA). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:500-1:2000 IHC:1:50-1:200 IP:1:200-1:2000 |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMPD3 |
|---|---|
| Gene Synonyms | NSMASE2 |
| Gene Full Name | sphingomyelin phosphodiesterase 3 |
| Gene Summary | Enables sphingomyelin phosphodiesterase activity. Involved in sphingomyelin catabolic process. Predicted to be located in plasma membrane. Predicted to be active in cytoplasm. Biomarker of pulmonary emphysema. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Cca1 antibody Confluent 3Y1 cell-associated protein 1 antibody Neutral sphingomyelinase 2 antibody Neutral sphingomyelinase II antibody NSMA2_HUMAN antibody nSMase-2 antibody nSMase2 antibody SMPD3 antibody Sphingomyelin phosphodiesterase 3 antibody sphingomyelin phosphodiesterase 3 neutral membrane (neutral sphingomyelinase II) antibody |
| Molecular Weight(MW) | 71kDa |
| Cellular Localization | Golgi apparatus membrane,Cell membrane. |
IHC
Immunohistochemistry of paraffin-embedded human pancreatic cancer using SMPD3 Antibody at dilution of 1:100.
IP
Immunoprecipitating SMPD3 in mouse brain whole cell lysate, Lane 1: Rabbit control IgG (1μg) instead of SMPD3 Antibody in mouse brain whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: SMPD3 Antibody (6μg) + Mouse brain whole cell lysate (500μg), Lane 3: Mouse brain whole cell lysate (10μg).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 IP:1:200-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
