IHC
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-P2Y12 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-P2Y12 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-P2Y12 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-P
Species: Mouse, Site: Cerebral cortex, Sample: Paraffin-embedded section, Antibody concentration: 1:500.IF-F
Species: Mouse, Site: Cerebral cortex, Sample: Frozen section, Antibody concentration: 1:2,000, Antigen retrieval: Not required.IF-F
Species: Rat, Site: Cerebral cortex, Sample: Frozen section, Antibody concentration: 1:2,000, Antigen retrieval: Not required.| Product Name | P2Y12 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIF-PIHC |
| IHC:1:1000 IF-P:1:500 IF-F:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | P2RY12 |
|---|---|
| Gene Synonyms | HORK3 P2Y12 ADPG-R BDPLT8 SP1999 P2T(AC) P2Y(AC) P2Y(12)R P2Y(ADP) P2Y(cyc) |
| Gene Full Name | purinergic receptor P2Y12 |
| Gene Summary | The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is involved in platelet aggregation, and is a potential target for the treatment of thromboembolisms and other clotting disorders. Mutations in this gene are implicated in bleeding disorder, platelet type 8 (BDPLT8). Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013] |
| Alternative Names | ADP glucose receptor antibody ADP-glucose receptor antibody ADPG R antibody ADPG-R antibody ADPGR antibody BDPLT8 antibody G protein coupled receptor SP1999 antibody Gi coupled ADP receptor HORK 3 antibody Gi coupled ADP receptor HORK3 antibody HORK 3 antibody ADP glucose receptor antibody ADP-glucose receptor antibody ADPG R antibody ADPG-R antibody ADPGR antibody BDPLT8 antibody G protein coupled receptor SP1999 antibody Gi coupled ADP receptor HORK 3 antibody Gi coupled ADP receptor HORK3 antibody HORK 3 antibody HORK3 antibody P2RY 12 antibody P2RY12 antibody P2T(AC) antibody P2Y 12 antibody P2Y purinoceptor 12 antibody P2Y(12)R antibody P2Y(AC) antibody P2Y(ADP) antibody P2Y(cyc) antibody P2Y12 antibody P2Y12 platelet ADP receptor antibody P2Y12_HUMAN antibody Platelet ADP receptor antibody Purinergic receptor P2RY12 antibody Purinergic receptor P2Y G protein coupled 12 antibody Purinergic receptor P2Y12 antibody Putative G protein coupled receptor antibody SP 1999 antibody SP1999 antibody |
| Molecular Weight(MW) | 39kDa |
| Cellular Localization | Cell membrane. |
IHC
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-P2Y12 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-P2Y12 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-P2Y12 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-P
Species: Mouse, Site: Cerebral cortex, Sample: Paraffin-embedded section, Antibody concentration: 1:500.
IF-F
Species: Mouse, Site: Cerebral cortex, Sample: Frozen section, Antibody concentration: 1:2,000, Antigen retrieval: Not required.
IF-F
Species: Rat, Site: Cerebral cortex, Sample: Frozen section, Antibody concentration: 1:2,000, Antigen retrieval: Not required.| Application Notes | IHC:1:1000 IF-P:1:500 IF-F:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
