WB
Western blot analysis of anti-RRP7A antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A341 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RRP7A antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of RRP7A using anti-RRP7A antibody. RRP7A was detected in a paraffin-embedded section of human testicular seminoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
IF analysis of RRP7A using anti-RRP7A antibody and anti-Tubulin beta antibody. RRP7A was detected in an immunocytochemical section of MCF-7 cells. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) were used as secondary antibody.FC
Flow Cytometry analysis of SiHa cells using anti-RRP7A antibody. Overlay histogram showing SiHa cells stained with anti-RRP7A antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RRP7A Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to RRP7A |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human RRP7A recombinant protein (Position: M1-D181). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMonkey |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RRP7A |
|---|---|
| Gene Synonyms | Rrp7 CGI-96 MCPH28 BK126B4.3 |
| Gene Full Name | ribosomal RNA processing 7 homolog A |
| Gene Summary | Enables RNA binding activity. Involved in cilium disassembly; protein localization to nucleolus; and ribosome biogenesis. Located in several cellular components, including centrosome; cilium; and nuclear lumen. Part of small-subunit processome. Implicated in primary autosomal recessive microcephaly 28. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | RRP7A CGI-96 Ribosomal RNA-processing protein 7 homolog A Gastric cancer antigen Zg14 |
| Molecular Weight(MW) | 37 kDa |
| Cellular Localization | Cell projection,Cytoplasm,Cytoskeleton,Nucleus. |
WB
Western blot analysis of anti-RRP7A antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A341 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RRP7A antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of RRP7A using anti-RRP7A antibody. RRP7A was detected in a paraffin-embedded section of human testicular seminoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
IF analysis of RRP7A using anti-RRP7A antibody and anti-Tubulin beta antibody. RRP7A was detected in an immunocytochemical section of MCF-7 cells. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) were used as secondary antibody.
FC
Flow Cytometry analysis of SiHa cells using anti-RRP7A antibody. Overlay histogram showing SiHa cells stained with anti-RRP7A antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RRP7A Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
