WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PERK (Phospho Thr980) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.| Product Name | PERK (Phospho Thr980) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-Thr980 |
| Modification Notes | PERK (Phospho Thr980) Antibody detects endogenous levels of PERK protein only when phosphorylated at T980.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):YArHT |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | EIF2AK3 |
|---|---|
| Gene Synonyms | PEK WRS PERK |
| Gene Full Name | eukaryotic translation initiation factor 2 alpha kinase 3 |
| Gene Summary | The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015] |
| Alternative Names | EIF2AK3 PEK PERK Eukaryotic translation initiation factor 2-alpha kinase 3 PRKR-like endoplasmic reticulum kinase Pancreatic eIF2-alpha kinase HsPEK |
| Molecular Weight(MW) | 125kDa |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type I membrane protein. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PERK (Phospho Thr980) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
