WB
Various lysates were subjected to SDS PAGE followed by western blot with TIM3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using TIM3 antibody at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using TIM3 antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | TIM3 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human HAVCR2 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:500-1:2000 IHC:1:500-1:2000 IF-P:1:50-1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.7mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HAVCR2 |
|---|---|
| Gene Synonyms | TIM3 CD366 KIM-3 SPTCL TIMD3 Tim-3 TIMD-3 HAVcr-2 |
| Gene Full Name | hepatitis A virus cellular receptor 2 |
| Gene Summary | The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011] |
| Alternative Names | CD366 HAVCR2 HAVcr-2 T-cell immunoglobulin and mucin domain-containing protein 3 T-cell immunoglobulin mucin receptor 3 |
| Molecular Weight(MW) | 50-70 kDa |
| Cellular Localization | Cell membrane,Cell junction. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with TIM3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using TIM3 antibody at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using TIM3 antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:500-1:2000 IHC:1:500-1:2000 IF-P:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
