WB
Western blot analysis of lysates from iBMDM cells using Cleaved Gasdermin D (C Terminal) Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. iBMDM cells were treated with LPS(100 ng/mL) at 37℃ for 4 hours and Nigericin(20μM) at 37℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1 s.IP
Immunoprecipitation of Cleaved Gasdermin D (C Terminal) from 600 µg extracts of iBMDM cells treated with LPS(100ng/ml, 4h) and Nigericin(15uM, 30min) was performed using 1 µg of Cleaved Gasdermin D (C Terminal) Rabbit mAb . Rabbit Control IgG was used to precipitate the Control IgG sample. IP samples were eluted with 1x reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Cleaved Gasdermin D (C Terminal) Rabbit mAb at a dilution of 1:5000.| Product Name | Cleaved Gasdermin D (C Terminal) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| WB:1:5000-1:10000 IP:0.5μg-4μg antibody for 500μg-700μg extracts of whole cells |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Gsdmd |
|---|---|
| Gene Synonyms | DF5L M2-4 Dfna5l GsdmD-1 Gsdmdc1 1810036L03Rik |
| Gene Full Name | Enables phospholipid binding activity; transcription coactivator activity; and wide pore channel activity. Involved in several processes, including defense response to bacterium; positive regulation of interleukin-1 beta production; and protein-containing complex assembly. Acts upstream of or within ceramide biosynthetic process; plasma membrane repair; and protein maturation. Located in cytosol; extracellular space; and mitochondrial membrane. Part of NLRP3 inflammasome complex. Is active in plasma membrane. Is expressed in several structures, including aorta; gut; liver; metanephros; and spleen. Orthologous to human GSDMD (gasdermin D). [provided by Alliance of Genome Resources, Apr 2025] |
| Alternative Names | DF5L M2-4 Dfna5l GsdmD-1 Gsdmdc1 1810036L03Rik |
| Molecular Weight(MW) | 53kDa(Observed MW 22kDa) |
| Cellular Localization | cytosol, mitochondrial membrane, extracellular space, NLRP3 inflammasome complex, nucleoplasm, plasma membrane. |
WB
Western blot analysis of lysates from iBMDM cells using Cleaved Gasdermin D (C Terminal) Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. iBMDM cells were treated with LPS(100 ng/mL) at 37℃ for 4 hours and Nigericin(20μM) at 37℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1 s.
IP
Immunoprecipitation of Cleaved Gasdermin D (C Terminal) from 600 µg extracts of iBMDM cells treated with LPS(100ng/ml, 4h) and Nigericin(15uM, 30min) was performed using 1 µg of Cleaved Gasdermin D (C Terminal) Rabbit mAb . Rabbit Control IgG was used to precipitate the Control IgG sample. IP samples were eluted with 1x reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Cleaved Gasdermin D (C Terminal) Rabbit mAb at a dilution of 1:5000.| Application Notes | WB:1:5000-1:10000 IP:0.5μg-4μg antibody for 500μg-700μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
