WB
Western Blot analysis of various cells using Brp44L Polyclonal Antibody.WB
Western blot analysis of lysates from COLO and HeLa cells, using BRP44L Antibody. The lane on the right is blocked with the synthesized peptide.IHC
Immunohistochemistry analysis of paraffin-embedded human heart tissue, using BRP44L Antibody. The picture on the right is blocked with the synthesized peptide.ICC/IF
Immunofluorescence analysis of Hela cell. 1,Brp44L Polyclonal Antibody(green) was diluted at 1:200(4° overnight). (red) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min).| Product Name | Brp44L Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human BRP44L. AA range:11-60 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MPC1 |
|---|---|
| Gene Synonyms | MPYCD BRP44L CGI-129 SLC54A1 |
| Gene Full Name | mitochondrial pyruvate carrier 1 |
| Gene Summary | The protein encoded by this gene is part of an MPC1/MPC2 heterodimer that is responsible for transporting pyruvate into mitochondria. The encoded protein is found in the inner mitochondrial membrane. Defects in this gene are a cause of mitochondrial pyruvate carrier deficiency. Several transcript variants, some protein coding and one non-protein coding, have been found for this gene. [provided by RefSeq, Aug 2012] |
| Alternative Names | MPC1 BRP44L CGI-129 HSPC040 PNAS-115 Mitochondrial pyruvate carrier 1 Brain protein 44-like protein |
| Molecular Weight(MW) | 12kDa |
| Cellular Localization | Mitochondrion inner membrane ; Multi-pass membrane protein . |
WB
Western Blot analysis of various cells using Brp44L Polyclonal Antibody.
WB
Western blot analysis of lysates from COLO and HeLa cells, using BRP44L Antibody. The lane on the right is blocked with the synthesized peptide.
IHC
Immunohistochemistry analysis of paraffin-embedded human heart tissue, using BRP44L Antibody. The picture on the right is blocked with the synthesized peptide.
ICC/IF
Immunofluorescence analysis of Hela cell. 1,Brp44L Polyclonal Antibody(green) was diluted at 1:200(4° overnight). (red) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min).| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
