WB
HeLa cells were subjected to SDS PAGE followed by western blot with MFAP5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using MFAP5 antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | MFAP5 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human MFAP5 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:4000 IHC:1:400-1:1600 |
|
| Species Reactivity | Human |
| Concentration | 0.55mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MFAP5 |
|---|---|
| Gene Synonyms | AAT9 MP25 MAGP2 MAGP-2 MFAP-5 |
| Gene Full Name | microfibril associated protein 5 |
| Gene Summary | This gene encodes a 25-kD microfibril-associated glycoprotein which is a component of microfibrils of the extracellular matrix. The encoded protein promotes attachment of cells to microfibrils via alpha-V-beta-3 integrin. Deficiency of this gene in mice results in neutropenia. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014] |
| Alternative Names | MAGP 2 MAGP2 MAGP-2 MFAP 5 MFAP-5 |
| Molecular Weight(MW) | 20-25kDa |
| Cellular Localization | Extracellular matrix,Secreted. |
WB
HeLa cells were subjected to SDS PAGE followed by western blot with MFAP5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using MFAP5 antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:1000-1:4000 IHC:1:400-1:1600 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
