WB
Western blot analysis of DNAM-1/CD226 using anti-DNAM-1/CD226 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse thymus tissue lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DNAM-1/CD226 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
DNAM-1/CD226 was detected in a paraffin-embedded section of mouse lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-DNAM-1/CD226 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
DNAM-1/CD226 was detected in a paraffin-embedded section of rat lung lymphaden tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-DNAM-1/CD226 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.FC
Flow Cytometry analysis of NRK cells using anti-DNAM-1/CD226 antibody. Overlay histogram showing NRK cells stained with anti-DNAM-1/CD226 antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DNAM-1/CD226 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to DNAM-1/CD226 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived mouse CD226 recombinant protein (Position: E31-L333). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | MouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Cd226 |
|---|---|
| Gene Synonyms | Pta1 DNAM1 DNAM-1 TLiSA1 |
| Gene Full Name | CD226 antigen |
| Gene Summary | Predicted to enable identical protein binding activity; integrin binding activity; and protein kinase binding activity. Acts upstream of or within positive regulation of natural killer cell cytokine production and positive regulation of type II interferon production. Located in external side of plasma membrane. Is expressed in embryo. Orthologous to human CD226 (CD226 molecule). [provided by Alliance of Genome Resources, Apr 2025] |
| Alternative Names | CD226 CD226 antigen CD226 molecule DNAM 1 DNAM1 DNAX accessory molecule 1 PTA1 TLiSA1 |
| Molecular Weight(MW) | 70kDa |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of DNAM-1/CD226 using anti-DNAM-1/CD226 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse thymus tissue lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DNAM-1/CD226 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
DNAM-1/CD226 was detected in a paraffin-embedded section of mouse lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-DNAM-1/CD226 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
DNAM-1/CD226 was detected in a paraffin-embedded section of rat lung lymphaden tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-DNAM-1/CD226 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
FC
Flow Cytometry analysis of NRK cells using anti-DNAM-1/CD226 antibody. Overlay histogram showing NRK cells stained with anti-DNAM-1/CD226 antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DNAM-1/CD226 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
