WB
Western blot analysis of LKB1 on different lysates with Rabbit anti-LKB1 antibody at 1/2,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane), Lane 2: Jurkat cell lysate (20 µg/Lane), Lane 3: HeLa cell lysate (negative) (20 µg/Lane), Lane 4: HEK-293 cell lysate (20 µg/Lane), Lane 5: COS-1 cell lysate (20 µg/Lane), Lane 6: Mouse brain tissue lysate (40 µg/Lane), Lane 7: Mouse testis tissue lysate (40 µg/Lane), Lane 8: Rat testis tissue lysate (40 µg/Lane), Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-LKB1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-LKB1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-LKB1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IP
LKB1 was immunoprecipitated from 0.2 mg HEK-293 cell lysate with Rabbit anti-LKB1 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-LKB1 antibody at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HEK-293 cell lysate (input), Lane 2: Rabbit anti-LKB1 antibody IP in HEK-293 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-LKB1 antibody in HEK-293 cell lysate. Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes.| Product Name | LKB1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human LKB1 aa 1-400. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:2000 IHC:1:200-1:1000 IP:1-2μg/sample |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | STK11 |
|---|---|
| Gene Synonyms | PJS LKB1 hLKB1 |
| Gene Full Name | serine/threonine kinase 11 |
| Gene Summary | The protein encoded by this gene is a serine/threonine kinase that regulates cell polarity and energy metabolism and functions as a tumor suppressor. Mutations in this gene have been associated with the autosomal dominant Peutz-Jeghers syndrome, as well as with skin, pancreatic, and testicular cancers. [provided by RefSeq, May 2022] |
| Alternative Names | hLKB1 antibody Liver kinase B1 antibody LKB1 antibody PJS antibody Polarization related protein LKB1 antibody Renal carcinoma antigen NY-REN-19 antibody Serine/Threonine Kinase 11 antibody Serine/threonine protein kinase 11 antibody Serine/threonine protein kinase LKB1 antibody Serine/threonine protein kinase STK11 antibody hLKB1 antibody Liver kinase B1 antibody LKB1 antibody PJS antibody Polarization related protein LKB1 antibody Renal carcinoma antigen NY-REN-19 antibody Serine/Threonine Kinase 11 antibody Serine/threonine protein kinase 11 antibody Serine/threonine protein kinase LKB1 antibody Serine/threonine protein kinase STK11 antibody Serine/threonine-protein kinase 11 antibody Serine/threonine-protein kinase LKB1 antibody Serine/threonine-protein kinase XEEK1 antibody Stk11 antibody STK11_HUMAN antibody |
| Molecular Weight(MW) | 49kDa(Observed band size: 56/50kDa) |
| Cellular Localization | Nucleus, Cytoplasm, Membrane, Mitochondrion. |
WB
Western blot analysis of LKB1 on different lysates with Rabbit anti-LKB1 antibody at 1/2,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane), Lane 2: Jurkat cell lysate (20 µg/Lane), Lane 3: HeLa cell lysate (negative) (20 µg/Lane), Lane 4: HEK-293 cell lysate (20 µg/Lane), Lane 5: COS-1 cell lysate (20 µg/Lane), Lane 6: Mouse brain tissue lysate (40 µg/Lane), Lane 7: Mouse testis tissue lysate (40 µg/Lane), Lane 8: Rat testis tissue lysate (40 µg/Lane), Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-LKB1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-LKB1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-LKB1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IP
LKB1 was immunoprecipitated from 0.2 mg HEK-293 cell lysate with Rabbit anti-LKB1 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-LKB1 antibody at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HEK-293 cell lysate (input), Lane 2: Rabbit anti-LKB1 antibody IP in HEK-293 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-LKB1 antibody in HEK-293 cell lysate. Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes.| Application Notes | WB:1:2000 IHC:1:200-1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
