WB
Western blot analysis of Phospho-Tau (S202) on different lysates with Rabbit anti-Phospho-Tau (S202) antibody at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate,
Lane 2: Rat hippocampus tissue lysate,
Lane 3: Mouse brain treated with λpp for 1 hour tissue lysate,
Lysates/proteins at 30 µg/Lane.
Exposure time: 1 minute 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.