WB
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with CYP3A4 using CYP3A4 Rabbit mAb at1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.IF-P
Confocal imaging of paraffin-embedded Human liver tissue using CYP3A4 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.| Product Name | CYP3A4 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PWB |
| WB:1:2000-1:12000 IF-P:1:100-1:800 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CYP3A4 |
|---|---|
| Gene Synonyms | HLP CP33 CP34 CYP3A NF-25 VDDR3 CYP3A3 P450C3 CYPIIIA3 CYPIIIA4 P450PCN1 |
| Gene Full Name | cytochrome P450 family 3 subfamily A member 4 |
| Gene Summary | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam, erythromycin, and chloroquine. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2020] |
| Alternative Names | HLP CP33 CP34 CYP3A NF-25 VDDR3 CYP3A3 P450C3 CYPIIIA3 CYPIIIA4 P450PCN1 CYP3A4 |
| Molecular Weight(MW) | 57kDa |
| Cellular Localization | Endoplasmic reticulum membrane, Microsome membrane, Single-pass membrane protein. |
WB
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with CYP3A4 using CYP3A4 Rabbit mAb at1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
IF-P
Confocal imaging of paraffin-embedded Human liver tissue using CYP3A4 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.| Application Notes | WB:1:2000-1:12000 IF-P:1:100-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
