WB
Western blot analysis of various lysates, using PU.1/SPI1 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC):HAP1 Exposure time: 15s.IHC
Immunohistochemistry analysis of paraffin embedded Human appendix tissue using PU.1/SPI1 Rabbit mAb at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Human follicular lymphoma tissue using PU.1/SPI1 Rabbit mAb at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using PU.1/SPI1 Rabbit mAb at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.ICC/IF
Confocal imaging of THP-1 cells using PU.1/SPI1 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.IF-P
Confocal imaging of paraffin-embedded Human spleen tissue using PU.1/SPI1 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.FC
Flow cytometry: 1X10^6 HAP1 cells (negative control,left) and THP-1 cells (right) were intracellularly-stained with PU.1/SPI1 Rabbit mAb (2 μg/mL,orange line) or 594 Rabbit IgG isotype control (5 μl/Test,blue line), followed by 594-conjugated Goat Anti-Rabbit IgG (H+L) staining. Non-fluorescently stained cells were used as blank control (red line).| Product Name | PU.1/SPI1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PIHCWB |
| WB:1:1000-1:6000 IHC:1:500-1:5000 ICC/IF:1:50-1:200 IF-P:1:50-1:200 FC:1:100-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPI1 |
|---|---|
| Gene Synonyms | OF PU.1 AGM10 SFPI1 SPI-1 SPI-A |
| Gene Full Name | Spi-1 proto-oncogene |
| Gene Summary | This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | OF PU.1 AGM10 SFPI1 SPI-1 SPI-A PU.1/SPI1 |
| Molecular Weight(MW) | 31kDa(Observed MW 38kDa) |
| Cellular Localization | nucleoplasm, nucleus. |
WB
Western blot analysis of various lysates, using PU.1/SPI1 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC):HAP1 Exposure time: 15s.
IHC
Immunohistochemistry analysis of paraffin embedded Human appendix tissue using PU.1/SPI1 Rabbit mAb at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Human follicular lymphoma tissue using PU.1/SPI1 Rabbit mAb at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using PU.1/SPI1 Rabbit mAb at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ICC/IF
Confocal imaging of THP-1 cells using PU.1/SPI1 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
IF-P
Confocal imaging of paraffin-embedded Human spleen tissue using PU.1/SPI1 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
FC
Flow cytometry: 1X10^6 HAP1 cells (negative control,left) and THP-1 cells (right) were intracellularly-stained with PU.1/SPI1 Rabbit mAb (2 μg/mL,orange line) or 594 Rabbit IgG isotype control (5 μl/Test,blue line), followed by 594-conjugated Goat Anti-Rabbit IgG (H+L) staining. Non-fluorescently stained cells were used as blank control (red line).| Application Notes | WB:1:1000-1:6000 IHC:1:500-1:5000 ICC/IF:1:50-1:200 IF-P:1:50-1:200 FC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
