WB
Western blot analysis of Sorbitol Dehydrogenase/SORD using anti-Sorbitol Dehydrogenase/SORD antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Human HepG2 whole cell lysates, Lane 2: Human U937 whole cell lysates, Lane 3: Human K562 whole cell lysates, Lane 4: Human A431 whole cell lysates, Lane 5: Monkey kidney tissue lysates, Lane 6: Rat liver tissue lysates, Lane 7: Rat kidney tissue lysates, Lane 8: Mouse liver tissue lysates, Lane 9: Mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Sorbitol Dehydrogenase/SORD antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
Sorbitol Dehydrogenase/SORD was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Sorbitol Dehydrogenase/SORD Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
Sorbitol Dehydrogenase/SORD was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Sorbitol Dehydrogenase/SORD Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
Sorbitol Dehydrogenase/SORD was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-Sorbitol Dehydrogenase/SORD Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Overlay histogram showing Caco-2 cells stained with anti-Sorbitol Dehydrogenase/SORD antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Sorbitol Dehydrogenase/SORD Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to Sorbitol Dehydrogenase/SORD |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human Sorbitol Dehydrogenase/SORD recombinant protein (Position: N8-P357). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SORD |
|---|---|
| Gene Synonyms | RDH SDH XDH HMNR8 SORD1 SORDD HEL-S-95n |
| Gene Full Name | sorbitol dehydrogenase |
| Gene Summary | Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010] |
| Alternative Names | L iditol 2 dehydrogenase SDH Sorbitol dehydrogenase SORD SORD1 |
| Molecular Weight(MW) | 38 kDa(Observed band size: 43kDa) |
| Cellular Localization | Cell projection,Cilium,Flagellum,Membrane,Mitochondrion. |
WB
Western blot analysis of Sorbitol Dehydrogenase/SORD using anti-Sorbitol Dehydrogenase/SORD antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Human HepG2 whole cell lysates, Lane 2: Human U937 whole cell lysates, Lane 3: Human K562 whole cell lysates, Lane 4: Human A431 whole cell lysates, Lane 5: Monkey kidney tissue lysates, Lane 6: Rat liver tissue lysates, Lane 7: Rat kidney tissue lysates, Lane 8: Mouse liver tissue lysates, Lane 9: Mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Sorbitol Dehydrogenase/SORD antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
Sorbitol Dehydrogenase/SORD was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Sorbitol Dehydrogenase/SORD Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
Sorbitol Dehydrogenase/SORD was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Sorbitol Dehydrogenase/SORD Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
Sorbitol Dehydrogenase/SORD was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-Sorbitol Dehydrogenase/SORD Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Overlay histogram showing Caco-2 cells stained with anti-Sorbitol Dehydrogenase/SORD antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Sorbitol Dehydrogenase/SORD Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
