WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MDM2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A431, Lane 2: C2C12, Lane 3: Raji.IHC
Human placenta was stained with anti-MDM2 rabbit antibody.IHC
Rat lung was stained with anti-MDM2 rabbit antibody.ICC/IF
Immunofluorescence analysis of Hela cell. 1,MDM2 Monoclonal Antibody(red) was diluted at 1:200(4° overnight). LC3B Monoclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4～C, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: Hela - Human cervical cancer, Lane2: HEK293 - Human normal embryonic kidney, Lane3: HepG2 - Human hepatocellular carcinoma, Lane4: HCT-116 - Human colon cancer, Lane5: A549 - Human lung adenocarcinoma, Lane6: A431 - Human skin squamous cell carcinoma, Lane7: K562 - Human chronic myeloid leukemia, Lane8: Jurkat - Human acute T cell leukemia cells, Lane9: MCF7 - Human breast cancer, Lane10: SH-SY5Y - Human neuroblastoma cells, Lane11: NIH-3T3 - NIH mouse fibroblasts, Lane12: RAW264.7 - Mouse mononuclear macrophage leukemia cells, Lane13: C6 - Rat glioma cells, Lane14: PC-12 - Pheochromocytoma in rats.| Product Name | MDM2 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:5000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MDM2 |
|---|---|
| Gene Synonyms | HDMX LSKB hdm2 ACTFS |
| Gene Full Name | MDM2 proto-oncogene |
| Gene Summary | This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013] |
| Alternative Names | MDM2 E3 ubiquitin-protein ligase Mdm2 Double minute 2 protein Hdm2 Oncoprotein Mdm2 p53-binding protein Mdm2 |
| Molecular Weight(MW) | 55kDa(Observed 90kDa) |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MDM2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A431, Lane 2: C2C12, Lane 3: Raji.
IHC
Human placenta was stained with anti-MDM2 rabbit antibody.
IHC
Rat lung was stained with anti-MDM2 rabbit antibody.
ICC/IF
Immunofluorescence analysis of Hela cell. 1,MDM2 Monoclonal Antibody(red) was diluted at 1:200(4° overnight). LC3B Monoclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4～C, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: Hela - Human cervical cancer, Lane2: HEK293 - Human normal embryonic kidney, Lane3: HepG2 - Human hepatocellular carcinoma, Lane4: HCT-116 - Human colon cancer, Lane5: A549 - Human lung adenocarcinoma, Lane6: A431 - Human skin squamous cell carcinoma, Lane7: K562 - Human chronic myeloid leukemia, Lane8: Jurkat - Human acute T cell leukemia cells, Lane9: MCF7 - Human breast cancer, Lane10: SH-SY5Y - Human neuroblastoma cells, Lane11: NIH-3T3 - NIH mouse fibroblasts, Lane12: RAW264.7 - Mouse mononuclear macrophage leukemia cells, Lane13: C6 - Rat glioma cells, Lane14: PC-12 - Pheochromocytoma in rats.| Application Notes | WB:1:1000-1:5000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
