WB
Positive WB detected in: HL60 whole cell lysate(20µg),Mouse Spleen tissue lysate(20µg), All lanes: KLRG1 antibody at 1:1000, Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution.ICC/IF
Immunofluorescence staining of A549 cell with KLRG1 Antibody at 1:70, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).ICC/IF
Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-KLRG1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Killer cell lectin-like receptor subfamily G member 1 protein (60-195AA)). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KLRG1 |
|---|---|
| Gene Synonyms | 2F1 MAFA MAFA-L CLEC15A MAFA-2F1 MAFA-LIKE |
| Gene Full Name | killer cell lectin like receptor G1 |
| Gene Summary | Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. The protein encoded by this gene belongs to the killer cell lectin-like receptor (KLR) family, which is a group of transmembrane proteins preferentially expressed in NK cells. Studies in mice suggested that the expression of this gene may be regulated by MHC class I molecules. [provided by RefSeq, Jun 2016] |
| Alternative Names | 2F1 Ag antibody 2F1 antibody C type lectin domain family 15 member A antibody C-type lectin domain family 15 member A antibody CLEC15A antibody ITIM containing receptor MAFA L antibody ITIM-containing receptor MAFA-L antibody Killer cell lectin like receptor G1 antibody Killer cell lectin like receptor subfamily G member 1 antibody Killer cell lectin-like receptor subfamily G member 1 antibody KLRG 1 antibody KLRG1 antibody KLRG1 protein antibody KLRG1_HUMAN antibody MAFA 2F1 antibody MAFA antibody MAFA L antibody MAFA like antibody MAFA like receptor antibody MAFA-like receptor antibody MAFAL antibody Mast cell function associated antigen (ITIM containing) antibody Mast cell function associated antigen antibody Mast cell function-associated antigen 2F1 antibody Mast cell function-associated antigen antibody Mast cell function-associated antigen rat homolog of antibody MGC13600 antibody |
| Molecular Weight(MW) | 38kDa |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein. |
WB
Positive WB detected in: HL60 whole cell lysate(20µg),Mouse Spleen tissue lysate(20µg), All lanes: KLRG1 antibody at 1:1000, Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution.
ICC/IF
Immunofluorescence staining of A549 cell with KLRG1 Antibody at 1:70, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
ICC/IF
Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
