WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: Hela - Human cervical cancer Lane,2: HEK293 - Human normal embryonic kidney, Lane3: HepG2 - Human hepatocellular carcinoma,Lane4: HCT-116 - Human colon cancer, Lane5: A549 - Human lung adenocarcinoma, Lane6: A431 - Human skin squamous cell carcinoma, Lane7: K562 - Human chronic myeloid leukemia, Lane8: Jurkat - Human acute T cell leukemia cells, Lane9: MCF7 - Human breast cancer, Lane10: SH-SY5Y - Human neuroblastoma cells, Lane11: NIH-3T3 - NIH mouse fibroblasts, Lane12: RAW264.7 - Mouse mononuclear macrophage leukemia cells, Lane13: C6 - Rat glioma cells, Lane14: PC-12 - Pheochromocytoma in rats.IHC
Human testis was stained with anti-TBK1 rabbit antibody.IHC
Rat liver was stained with anti-TBK1 rabbit antibody.ICC/IF
Immunofluorescence analysis of HEK293. Picture A: TBK1 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Product Name | TBK1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:5000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TBK1 |
|---|---|
| Gene Synonyms | NAK T2K AIARV IIAE8 FTDALS4 |
| Gene Full Name | TANK binding kinase 1 |
| Gene Summary | The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. The protein is also an important kinase for antiviral innate immunity response. [provided by RefSeq, Sep 2021] |
| Alternative Names | TBK1 NAK Serine/threonine-protein kinase TBK1 NF-kappa-B-activating kinase T2K TANK-binding kinase 1 |
| Molecular Weight(MW) | 84kDa |
| Cellular Localization | Cytoplasm. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: Hela - Human cervical cancer Lane,2: HEK293 - Human normal embryonic kidney, Lane3: HepG2 - Human hepatocellular carcinoma,Lane4: HCT-116 - Human colon cancer, Lane5: A549 - Human lung adenocarcinoma, Lane6: A431 - Human skin squamous cell carcinoma, Lane7: K562 - Human chronic myeloid leukemia, Lane8: Jurkat - Human acute T cell leukemia cells, Lane9: MCF7 - Human breast cancer, Lane10: SH-SY5Y - Human neuroblastoma cells, Lane11: NIH-3T3 - NIH mouse fibroblasts, Lane12: RAW264.7 - Mouse mononuclear macrophage leukemia cells, Lane13: C6 - Rat glioma cells, Lane14: PC-12 - Pheochromocytoma in rats.
IHC
Human testis was stained with anti-TBK1 rabbit antibody.
IHC
Rat liver was stained with anti-TBK1 rabbit antibody.
ICC/IF
Immunofluorescence analysis of HEK293. Picture A: TBK1 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Application Notes | WB:1:1000-1:5000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
