WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4～C, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: Hela - Human cervical cancer Lane2: HEK293 - Human normal embryonic kidney Lane3: HepG2 - Human hepatocellular carcinoma Lane4: HCT-116 - Human colon cancer Lane5: A549 - Human lung adenocarcinoma Lane6: A431 - Human skin squamous cell carcinoma Lane7: K562 - Human chronic myeloid leukemia Lane8: Jurkat - Human acute T cell leukemia cells Lane9: MCF7 - Human breast cancer Lane10: SH-SY5Y - Human neuroblastoma cells Lane11: NIH-3T3 - NIH mouse fibroblasts Lane12: RAW264.7 - Mouse mononuclear macrophage leukemia cells Lane13: C6 - Rat glioma cells Lane14: PC-12 - Pheochromocytoma in rats.IHC
Human brain was stained with anti-RPS6 rabbit antibody.IHC
Mouse brain was stained with anti-RPS6 rabbit antibody.IHC
Rat brain was stained with anti-RPS6 rabbit antibody.ICC/IF
Immunofluorescence analysis of HEK293. Picture A: RPS6 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Product Name | RPS6 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:5000 IHC:1:1000-1:5000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RPS6 |
|---|---|
| Gene Synonyms | S6 eS6 |
| Gene Full Name | ribosomal protein S6 |
| Gene Summary | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] |
| Alternative Names | RPS6 OK/SW-cl.2 40S ribosomal protein S6 Phosphoprotein NP33 |
| Molecular Weight(MW) | 31kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4～C, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: Hela - Human cervical cancer Lane2: HEK293 - Human normal embryonic kidney Lane3: HepG2 - Human hepatocellular carcinoma Lane4: HCT-116 - Human colon cancer Lane5: A549 - Human lung adenocarcinoma Lane6: A431 - Human skin squamous cell carcinoma Lane7: K562 - Human chronic myeloid leukemia Lane8: Jurkat - Human acute T cell leukemia cells Lane9: MCF7 - Human breast cancer Lane10: SH-SY5Y - Human neuroblastoma cells Lane11: NIH-3T3 - NIH mouse fibroblasts Lane12: RAW264.7 - Mouse mononuclear macrophage leukemia cells Lane13: C6 - Rat glioma cells Lane14: PC-12 - Pheochromocytoma in rats.
IHC
Human brain was stained with anti-RPS6 rabbit antibody.
IHC
Mouse brain was stained with anti-RPS6 rabbit antibody.
IHC
Rat brain was stained with anti-RPS6 rabbit antibody.
ICC/IF
Immunofluorescence analysis of HEK293. Picture A: RPS6 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Application Notes | WB:1:1000-1:5000 IHC:1:1000-1:5000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
