WB
Western blot analysis of SLC39A10 using anti-SLC39A10 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SLC39A10 antigA03957-Aen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
SLC39A10 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-SLC39A10 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
SLC39A10 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-SLC39A10 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
SLC39A10 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-SLC39A10 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
SLC39A10 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-SLC39A10 Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).IF-P
SLC39A10 was detected in a paraffin-embedded section of human thyroid cancer tissue. The tissue section was incubated with rabbit anti-SLC39A10 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).IF-P
SLC39A10 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-SLC39A10 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).IF-P
SLC39A10 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-SLC39A10 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Overlay histogram showing Caco-2 cells stained with anti-SLC39A10 Antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC39A10 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to SLC39A10 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human SLC39A10 recombinant protein (Position: H29-H379). Human SLC39A10 shares 77.9% amino acid (aa) sequence identity with mouse SLC39A10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC39A10 |
|---|---|
| Gene Synonyms | ZIP10 LZT-Hs2 |
| Gene Full Name | solute carrier family 39 member 10 |
| Gene Summary | Zinc is an essential cofactor for hundreds of enzymes. It is involved in protein, nucleic acid, carbohydrate, and lipid metabolism, as well as in the control of gene transcription, growth, development, and differentiation. SLC39A10 belongs to a subfamily of proteins that show structural characteristics of zinc transporters (Taylor and Nicholson, 2003 [PubMed 12659941]).[supplied by OMIM, Mar 2008] |
| Alternative Names | Zinc transporter ZIP10 Solute carrier family 39 member 10 Zrt- and Irt-like protein 10 ZIP-10 SLC39A10 KIAA1265 ZIP10 |
| Molecular Weight(MW) | 95-100 kDa |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of SLC39A10 using anti-SLC39A10 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SLC39A10 antigA03957-Aen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
SLC39A10 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-SLC39A10 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
SLC39A10 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-SLC39A10 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
SLC39A10 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-SLC39A10 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
SLC39A10 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-SLC39A10 Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IF-P
SLC39A10 was detected in a paraffin-embedded section of human thyroid cancer tissue. The tissue section was incubated with rabbit anti-SLC39A10 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IF-P
SLC39A10 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-SLC39A10 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IF-P
SLC39A10 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-SLC39A10 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Overlay histogram showing Caco-2 cells stained with anti-SLC39A10 Antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC39A10 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
