IHC
Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue with Rabbit anti-Orexin antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue with Rabbit anti-Orexin antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-F
Species: Mouse, Site: hypothalamus, Sample: Frozen section, Antibody concentration: 1/100, Antigen retrieval: Not required.| Product Name | Orexin Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIHC |
| IHC:1:100 IF-F:1:100 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Hcrt |
|---|---|
| Gene Synonyms | orexin-A |
| Gene Full Name | hypocretin neuropeptide precursor |
| Gene Summary | Enables neuropeptide hormone activity; type 1 orexin receptor binding activity; and type 2 orexin receptor binding activity. Involved in several processes, including excitatory postsynaptic potential; regulation of metal ion transport; and regulation of transmission of nerve impulse. Located in secretory granule. Is active in neuronal dense core vesicle lumen. Biomarker of hyperglycemia; obesity; and sleeping sickness. Human ortholog(s) of this gene implicated in heroin dependence and narcolepsy. Orthologous to human HCRT (hypocretin neuropeptide precursor). [provided by Alliance of Genome Resources, Apr 2025] |
| Alternative Names | HCRT antibody HCRT1 antibody HCRT2 antibody hypocretin (orexin) neuropeptide precursor antibody Hypocretin 1 antibody Hypocretin 2 antibody hypocretin antibody NRCLP1 antibody Orexin antibody Orexin A antibody HCRT antibody HCRT1 antibody HCRT2 antibody hypocretin (orexin) neuropeptide precursor antibody Hypocretin 1 antibody Hypocretin 2 antibody hypocretin antibody NRCLP1 antibody Orexin antibody Orexin A antibody Orexin B antibody Orexin precursor antibody OX antibody PPORX antibody PPOX antibody PREPROOREXIN antibody |
| Molecular Weight(MW) | 14 kDa |
| Cellular Localization | Rough endoplasmic reticulum, Cytoplasmic vesicle, Synapse. |
IHC
Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue with Rabbit anti-Orexin antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue with Rabbit anti-Orexin antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-F
Species: Mouse, Site: hypothalamus, Sample: Frozen section, Antibody concentration: 1/100, Antigen retrieval: Not required.| Application Notes | IHC:1:100 IF-F:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
