WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Src Family (Phospho Tyr416) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: NIH-3T3, Lane 2: NIH-3T3 was treated with PDGF(100 ng/mL) for 10 minutes, Lane 3: NIH-3T3, Lane 4: NIH-3T3 was treated with hPDGF-AA(50 ng/mL) for 30 minutes..| Product Name | Src Family (Phospho Tyr416) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-Tyr416 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SRC |
|---|---|
| Gene Synonyms | ASV SRC1 THC6 c-SRC p60-Src |
| Gene Full Name | SRC proto-oncogene, non-receptor tyrosine kinase |
| Gene Summary | This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Target | Phospho-Src (Y416) Antibody detects endogenous levels of c-Src protein only when phosphorylated at Y416.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):NEyTA |
| Alternative Names | SRC SRC1 Proto-oncogene tyrosine-protein kinase Src Proto-oncogene c-Src pp60c-src p60-Src ASV |
| Molecular Weight(MW) | 58-61kDa |
| Cellular Localization | Isoform 1: Membrane; Lipid anchor. Isoform 2: Membrane; Lipid anchor. Cytoplasm. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Src Family (Phospho Tyr416) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: NIH-3T3, Lane 2: NIH-3T3 was treated with PDGF(100 ng/mL) for 10 minutes, Lane 3: NIH-3T3, Lane 4: NIH-3T3 was treated with hPDGF-AA(50 ng/mL) for 30 minutes..| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
