WB
Western blot analysis of Phospho-PLC-gamma-1 (S1248) on different lysates with Rabbit anti-Phospho-PLC-gamma-1 (S1248) antibody at 1/2,000 dilution. Lane 1: A431 cell lysate, Lane 2: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate, Lane 3: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate, then the membrane treated with λpp for 1 hour, Lysates/proteins at 20 µg/Lane. Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperaturIP
Phospho-PLC-gamma-1 (S1248) was immunoprecipitated from 0.2 mg A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate with Rabbit anti-Phospho-PLC-gamma-1 (S1248) antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-Phospho-PLC-gamma-1 (S1248) antibody at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate (input), Lane 2: Rabbit anti-Phospho-PLC-gamma-1 (S1248) antibody IP in A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-Phospho-PLC-gamma-1 (S1248) antibody in A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 7 seconds.| Product Name | Phospho-PLC-gamma-1 (S1248) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Ser1248 of human PLC-gamma-1. |
| Modification | p-S1248 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| WB:1:2000-1:10000 IP:1-2μg/sample |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PLCG1 |
|---|---|
| Gene Synonyms | IDAA PLC1 NCKAP3 PLC-II PLC148 PLCgamma1 |
| Gene Full Name | phospholipase C gamma 1 |
| Gene Summary | The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody 1-phosphatidylinositol-4 5-bisphosphate phosphodiesterase gamma-1 antibody Inositoltrisphosphohydrolase antibody Monophosphatidylinositol phosphodiesterase antibody NCKAP3 antibody Phosphatidylinositol phospholipase C antibody Phosphoinositidase C antibody Phosphoinositide phospholipase C antibody Phosphoinositide phospholipase C-gamma-1 antibody 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody 1-phosphatidylinositol-4 5-bisphosphate phosphodiesterase gamma-1 antibody Inositoltrisphosphohydrolase antibody Monophosphatidylinositol phosphodiesterase antibody NCKAP3 antibody Phosphatidylinositol phospholipase C antibody Phosphoinositidase C antibody Phosphoinositide phospholipase C antibody Phosphoinositide phospholipase C-gamma-1 antibody Phospholipase C 148 antibody Phospholipase C gamma 1 antibody Phospholipase C-gamma-1 antibody Phospholipase C-II antibody PLC gamma 1 antibody PLC II antibody PLC-148 antibody PLC-gamma-1 antibody PLC-II antibody PLC1 antibody PLC148 antibody Plcg1 antibody PLCG1_HUMAN antibody PLCgamma1 antibody |
| Molecular Weight(MW) | 149 kDa(Observed band size: 149 kDa) |
| Cellular Localization | Cell projection, lamellipodium, ruffle. |
WB
Western blot analysis of Phospho-PLC-gamma-1 (S1248) on different lysates with Rabbit anti-Phospho-PLC-gamma-1 (S1248) antibody at 1/2,000 dilution. Lane 1: A431 cell lysate, Lane 2: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate, Lane 3: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate, then the membrane treated with λpp for 1 hour, Lysates/proteins at 20 µg/Lane. Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperatur
IP
Phospho-PLC-gamma-1 (S1248) was immunoprecipitated from 0.2 mg A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate with Rabbit anti-Phospho-PLC-gamma-1 (S1248) antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-Phospho-PLC-gamma-1 (S1248) antibody at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate (input), Lane 2: Rabbit anti-Phospho-PLC-gamma-1 (S1248) antibody IP in A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-Phospho-PLC-gamma-1 (S1248) antibody in A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 7 seconds.| Application Notes | WB:1:2000-1:10000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
