WB
Western blot analysis of NCR1 on NCR1 recombinant protein with Rabbit anti-NCR1 antibody at 1/5,000 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-NCR1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-NCR1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-F
Species: Mouse, Site: spleen, Sample: Frozen section, Antibody concentration: 1/500, Antigen retrieval: Not required.FC
Flow cytometric analysis of C57BL.6 mouse spleen cells labeling NCR1 and NK1.1-FITC. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (1/1,000). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 647 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃.| Product Name | NCR1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within mouse NCR1 aa 86-255. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF-FIHCWB |
| WB:1:5000 IHC:1:1000 IF-F:1:500 FC:1:1000 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Ncr1 |
|---|---|
| Gene Synonyms | Ly94 Cd335 NKp46 |
| Gene Full Name | natural cytotoxicity triggering receptor 1 |
| Gene Summary | Acts upstream of or within defense response to virus and detection of virus. Located in cell surface. Orthologous to human NCR1 (natural cytotoxicity triggering receptor 1). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Activating NK receptor NK p46 antibody CD335 antibody FLJ99094 antibody hNKp46 antibody Ly94 antibody Lymphocyte antigen 94 antibody Lymphocyte antigen 94 homolog (activating NK receptor NK p46) antibody Lymphocyte antigen 94 homolog antibody Natural cytotoxicity triggering receptor 1 antibody Activating NK receptor NK p46 antibody CD335 antibody FLJ99094 antibody hNKp46 antibody Ly94 antibody Lymphocyte antigen 94 antibody Lymphocyte antigen 94 homolog (activating NK receptor NK p46) antibody Lymphocyte antigen 94 homolog antibody Natural cytotoxicity triggering receptor 1 antibody Natural killer cell p46-related protein antibody NCR1 antibody NCT1 antibody NCTR1_HUMAN antibody NK cell activating receptor antibody NK cell-activating receptor antibody NK-p46 antibody NKp46 antibody |
| Molecular Weight(MW) | 37 kDa |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of NCR1 on NCR1 recombinant protein with Rabbit anti-NCR1 antibody at 1/5,000 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-NCR1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-NCR1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-F
Species: Mouse, Site: spleen, Sample: Frozen section, Antibody concentration: 1/500, Antigen retrieval: Not required.
FC
Flow cytometric analysis of C57BL.6 mouse spleen cells labeling NCR1 and NK1.1-FITC. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (1/1,000). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 647 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃.| Application Notes | WB:1:5000 IHC:1:1000 IF-F:1:500 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
