WB
Western blot analysis of CHRNA7 on different lysates with Rabbit anti-CHRNA7 antibody at 1/5,000 dilution. Lane 1: Jurkat cell lysate (no heat) (20 µg/Lane), Lane 2: HeLa cell lysate (no heat) (20 µg/Lane), Lane 3: A431 cell lysate (no heat) (20 µg/Lane), Lane 4: Caco-2 cell lysate (no heat) (20 µg/Lane), Lane 5: RAW264.7 cell lysate (no heat) (20 µg/Lane), Lane 6: C6 cell lysate (no heat) (20 µg/Lane), Lane 7: PC-12 cell lysate (no heat) (20 µg/Lane), Notice: no heat means the lysate is not boiled. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of CHRNA7 on different lysates with Rabbit anti-CHRNA7 antibody at 1/5,000 dilution. Lane 1: Jurkat cell lysate (no heat) (20 µg/Lane), Lane 2: C6 cell lysate (no heat) (20 µg/Lane), Lane 3: Mouse brain tissue lysate (no heat) (20 µg/Lane), Lane 4: Mouse spleen tissue lysate (no heat) (20 µg/Lane), Lane 5: Rat brain tissue lysate (no heat) (20 µg/Lane), Lane 6: Rat spleen tissue lysate (no heat) (20 µg/Lane), Notice: no heat means the lysate is not boiled. Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse dorsal root ganglion tissue with Rabbit anti-CHRNA7 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue with Rabbit anti-CHRNA7 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of Jurkat cells labeling CHRNA7 with Rabbit anti-CHRNA7 antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CHRNA7 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.IF-F
Species: Rat, Site: Kidney, Sample: Frozen section, Antibody concentration: 1/500, Antigen retrieval: Not required.FC
Flow cytometric analysis of Jurkat cells labeling CHRNA7. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | CHRNA7 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human CHRNA7 aa 290-502. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-FIHCWB |
| WB:1:5000 IHC:1:1000 ICC/IF:1:500 IF-F:1:500 FC:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CHRNA7 |
|---|---|
| Gene Synonyms | nAChR7 NACHRA7 CHRNA7-2 |
| Gene Full Name | cholinergic receptor nicotinic alpha 7 subunit |
| Gene Summary | The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012] |
| Alternative Names | 7nAChR antibody a7 nicotinic acetylcholine receptor antibody acetylcholine receptor neuronal nicotinic alpha-7 subunit antibody ACHA7_HUMAN antibody AChR antibody Acra7 antibody alpha 7 neuronal nicotinic acetylcholine receptor antibody alpha-7 nicotinic cholinergic receptor subunit antibody alpha7 antibody cholinergic receptor neuronal nicotinic alpha polypeptide 7 antibody 7nAChR antibody a7 nicotinic acetylcholine receptor antibody acetylcholine receptor neuronal nicotinic alpha-7 subunit antibody ACHA7_HUMAN antibody AChR antibody Acra7 antibody alpha 7 neuronal nicotinic acetylcholine receptor antibody alpha-7 nicotinic cholinergic receptor subunit antibody alpha7 antibody cholinergic receptor neuronal nicotinic alpha polypeptide 7 antibody cholinergic receptor nicotinic alpha 7 (neuronal) antibody cholinergic receptor nicotinic alpha 7 antibody cholinergic receptor nicotinic alpha polypeptide 7 antibody CHRNA7 antibody CHRNA7-2 antibody NACHRA7 antibody neuronal acetylcholine receptor protein alpha-7 chain antibody Neuronal acetylcholine receptor subunit alpha-7 antibody |
| Molecular Weight(MW) | 56 kDa(Observed band size: 60/48 kDa) |
| Cellular Localization | Postsynaptic cell membrane, cell membrane. |
WB
Western blot analysis of CHRNA7 on different lysates with Rabbit anti-CHRNA7 antibody at 1/5,000 dilution. Lane 1: Jurkat cell lysate (no heat) (20 µg/Lane), Lane 2: HeLa cell lysate (no heat) (20 µg/Lane), Lane 3: A431 cell lysate (no heat) (20 µg/Lane), Lane 4: Caco-2 cell lysate (no heat) (20 µg/Lane), Lane 5: RAW264.7 cell lysate (no heat) (20 µg/Lane), Lane 6: C6 cell lysate (no heat) (20 µg/Lane), Lane 7: PC-12 cell lysate (no heat) (20 µg/Lane), Notice: no heat means the lysate is not boiled. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of CHRNA7 on different lysates with Rabbit anti-CHRNA7 antibody at 1/5,000 dilution. Lane 1: Jurkat cell lysate (no heat) (20 µg/Lane), Lane 2: C6 cell lysate (no heat) (20 µg/Lane), Lane 3: Mouse brain tissue lysate (no heat) (20 µg/Lane), Lane 4: Mouse spleen tissue lysate (no heat) (20 µg/Lane), Lane 5: Rat brain tissue lysate (no heat) (20 µg/Lane), Lane 6: Rat spleen tissue lysate (no heat) (20 µg/Lane), Notice: no heat means the lysate is not boiled. Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse dorsal root ganglion tissue with Rabbit anti-CHRNA7 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue with Rabbit anti-CHRNA7 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of Jurkat cells labeling CHRNA7 with Rabbit anti-CHRNA7 antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CHRNA7 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
IF-F
Species: Rat, Site: Kidney, Sample: Frozen section, Antibody concentration: 1/500, Antigen retrieval: Not required.
FC
Flow cytometric analysis of Jurkat cells labeling CHRNA7. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:5000 IHC:1:1000 ICC/IF:1:500 IF-F:1:500 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
