WB
Positive WB detected in: HepG2 whole cell lysate, All lanes: LILRB3 antibody at 3.9µg/ml, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.ICC/IF
Immunofluorescence staining of NIH/3T3 cells with LILRB3 Antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-LILRB3 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Leukocyte immunoglobulin-like receptor subfamily B member 3 protein (470-624AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:5000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LILRB3 |
|---|---|
| Gene Synonyms | HL9 ILT5 LIR3 PIRB CD85A ILT-5 LIR-3 PIR-B LILRA6 |
| Gene Full Name | leukocyte immunoglobulin like receptor B3 |
| Gene Summary | This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | LILRB3 ILT5 LIR3 Leukocyte immunoglobulin-like receptor subfamily B member 3 LIR-3 Leukocyte immunoglobulin-like receptor 3 CD85 antigen-like family member A Immunoglobulin-like transcript 5 ILT-5 Monocyte inhibitory receptor HL9 CD antigen CD85a |
| Molecular Weight(MW) | 70 kDa |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. |
WB
Positive WB detected in: HepG2 whole cell lysate, All lanes: LILRB3 antibody at 3.9µg/ml, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.
ICC/IF
Immunofluorescence staining of NIH/3T3 cells with LILRB3 Antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:5000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
