WB
Western blot analysis of HEC1 on different lysates with Rabbit anti-HEC1 antibody at 1/1,000 dilution. Lane 1: K-562 cell lysate, Lane 2: U-2 OS cell lysate, Lane 3: HeLa cell lysate, Lane 4: HEK-293 cell lysate, Lane 5: Jurkat cell lysate, Lane 6: MCF7 cell lysate, Lane 7: HepG2 cell lysate, Lane 8: LO2 cell lysate, Lysates/proteins at 15 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.| Product Name | HEC1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human HEC1 aa 593-642 / 642. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NDC80 |
|---|---|
| Gene Synonyms | HEC HEC1 TID3 KNTC2 HsHec1 hsNDC80 |
| Gene Full Name | NDC80 kinetochore complex component |
| Gene Summary | This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011] |
| Alternative Names | from Entrez Gene antibody HEC antibody Highly expressed in cancer antibody Highly expressed in cancer protein antibody Highly expressed in cancer rich in leucine heptad repeats antibody HsHec1 antibody hsNDC80 antibody Kinetochore associated 2 antibody Kinetochore associated protein 2 antibody from Entrez Gene antibody HEC antibody Highly expressed in cancer antibody Highly expressed in cancer protein antibody Highly expressed in cancer rich in leucine heptad repeats antibody HsHec1 antibody hsNDC80 antibody Kinetochore associated 2 antibody Kinetochore associated protein 2 antibody Kinetochore protein Hec1 antibody Kinetochore protein NDC80 homolog antibody Kinetochore-associated protein 2 antibody KNTC2 antibody ndc80 antibody NDC80 homolog kinetochore complex component antibody NDC80 kinetochore complex component homolog antibody NDC80 S. cerevisiae homolog of antibody NDC80_HUMAN antibody Retinoblastoma associated protein HEC antibody Retinoblastoma-associated protein HEC antibody TID3 antibody |
| Molecular Weight(MW) | 74 kDa |
| Cellular Localization | Nucleus, Chromosome, centromere, kinetochore. |
WB
Western blot analysis of HEC1 on different lysates with Rabbit anti-HEC1 antibody at 1/1,000 dilution. Lane 1: K-562 cell lysate, Lane 2: U-2 OS cell lysate, Lane 3: HeLa cell lysate, Lane 4: HEK-293 cell lysate, Lane 5: Jurkat cell lysate, Lane 6: MCF7 cell lysate, Lane 7: HepG2 cell lysate, Lane 8: LO2 cell lysate, Lysates/proteins at 15 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
