WB
Western blot analysis of anti- SNF2H/SMARCA5 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates. Use rabbit anti- SNF2H/SMARCA5 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit.IHC
SMARCA5 was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. The tissue section was incubated with rabbit anti-SMARCA5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
SMARCA5 was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. The tissue section was incubated with rabbit anti-SMARCA5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
SMARCA5 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. The tissue section was incubated with rabbit anti-SMARCA5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
SMARCA5 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-SMARCA5 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) and Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (Green) were used as secondary antibody.FC
Overlay histogram showing THP-1 cells stained with anti-SMARCA5 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMARCA5 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to SMARCA5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human SNF2H/SMARCA5 recombinant protein (Position: A54-K735). Human SMARCA5 shares 99.1% amino acid (aa) sequence identity with mouse SMARCA5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMARCA5 |
|---|---|
| Gene Synonyms | ISWI SNF2H hISWI hSNF2H WCRF135 |
| Gene Full Name | SNF2 related chromatin remodeling ATPase 5 |
| Gene Summary | The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. [provided by RefSeq, Jul 2008] |
| Alternative Names | hISWI hSNF2H ISWI SMARCA5 SNF2H WCRF135 |
| Molecular Weight(MW) | 122 kDa |
| Cellular Localization | nucleus. |
WB
Western blot analysis of anti- SNF2H/SMARCA5 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates. Use rabbit anti- SNF2H/SMARCA5 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit.
IHC
SMARCA5 was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. The tissue section was incubated with rabbit anti-SMARCA5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
SMARCA5 was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. The tissue section was incubated with rabbit anti-SMARCA5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
SMARCA5 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. The tissue section was incubated with rabbit anti-SMARCA5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
SMARCA5 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-SMARCA5 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) and Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (Green) were used as secondary antibody.
FC
Overlay histogram showing THP-1 cells stained with anti-SMARCA5 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMARCA5 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
